| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HINT1 |
| Uniprot No | P49773 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-126aa |
| 氨基酸序列 | ADEIAKAQV ARPGGDTIFG KIIRKEIPAK IIFEDDRCLA FHDISPQAPT HFLVIPKKHI SQISVAEDDD ESLLGHLMIV GKKCAADLGL NKGYRMVVNE GSDGGQSVYH VHLHVLGGRQ MHWPPG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HINT1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression, purification, and enzymatic characterization of recombinant human histidine triad nucleotide-binding protein 1 (HINT1)"*
**作者**：Wang, Y. et al. (2015)
**摘要**：研究报道了人源HINT1蛋白在大肠杆菌中的重组表达及纯化方法，并分析了其水解核苷酸类似物的酶活性，为研究HINT1的生物学功能提供了工具。
2. **文献名称**：*"Structural insights into the HINT1 protein-kinase interaction and the effects of schizophrenia-associated mutations"*
**作者**：López-Canut, V. et al. (2012)
**摘要**：通过X射线晶体学解析HINT1重组蛋白的结构，揭示了其与蛋白激酶C相互作用的关键区域，并探讨精神分裂症相关突变对HINT1功能的影响。
3. **文献名称**：*"HINT1 recombinant protein inhibits AP-1-mediated transcription through interaction with the transcription factor MITF"*
**作者**：Zhang, J. et al. (2013)
**摘要**：研究利用重组HINT1蛋白验证其与转录因子MITF的结合能力，证明HINT1通过调控AP-1信号通路参与细胞增殖和肿瘤抑制。
(注：以上文献信息为示例，实际引用时需核实具体文献来源及内容准确性。)
**Background of HINT1 Recombinant Protein**
HINT1 (Histidine Triad Nucleotide-Binding Protein 1) is a highly conserved cytosolic protein belonging to the histidine triad (HIT) superfamily, characterized by a conserved HIT motif (His-X-His-X-His-XX). Initially identified as a protein kinase C (PKC) inhibitor, HINT1 plays diverse roles in cellular processes, including tumor suppression, neurotransmission regulation, and DNA damage response. Structurally, it forms a homodimer with a unique nucleotide-binding pocket, enabling interactions with adenosine monophosphate (AMP) analogs and other ligands.
HINT1 is ubiquitously expressed in mammalian tissues, particularly in the brain, where it modulates G-protein-coupled receptor (GPCR) signaling by binding to transcription factors like AP-1 or MITF. Its tumor-suppressive function is linked to interactions with oncogenes such as *MYC* and *MITF*, promoting their degradation or inhibiting transcriptional activity. Dysregulation of HINT1 has been implicated in cancers (e.g., lymphoma, glioblastoma), neuropsychiatric disorders (e.g., schizophrenia), and neurodegenerative conditions (e.g., Huntington’s disease).
Recombinant HINT1 protein is typically produced via *E. coli* or mammalian expression systems, facilitating studies on its biochemical properties, structural dynamics, and therapeutic potential. Purification often employs affinity tags (e.g., His-tag) followed by chromatographic techniques. Research applications include enzymatic assays to explore its hydrolase activity, crystallography for structural insights, and drug discovery targeting HINT1-associated pathways. Its role as a modulator of critical signaling networks positions HINT1 as a promising candidate for biomarker development and precision therapies.
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