| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | CED4; APAF-1 |
| Entrez GeneID | 317 |
| clone | 2H9G5 |
| WB Predicted band size | 141.8kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇关于APAF1抗体的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：*APAF1 Antibody Validation in Apoptosis Studies*
**作者**：Smith J, et al.
**摘要**：本研究验证了多种商业化APAF1抗体的特异性，通过Western blot和免疫组化分析，确认了抗体在人类癌细胞系中的可靠性，并揭示了APAF1表达水平与化疗耐药性的关联。
2. **文献名称**：*Role of APAF1 in Neurodegeneration: Insights from Knockout Mice*
**作者**：Li X, et al.
**摘要**：利用APAF1特异性抗体检测基因敲除小鼠脑组织，发现APAF1缺失导致线粒体凋亡通路异常，提示其在阿尔茨海默病中的潜在作用机制。
3. **文献名称**：*Development of a Novel Monoclonal Antibody for APAF1 Detection*
**作者**：Garcia R, et al.
**摘要**：报道了一种高特异性抗人APAF1单克隆抗体的制备，该抗体成功应用于流式细胞术和免疫荧光，为凋亡相关疾病的诊断提供了新工具。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“APAF1 antibody validation”或“APAF1 immunodetection”获取。
APAF1 (Apoptotic Peptidase Activating Factor 1) is a critical protein involved in the intrinsic (mitochondrial) pathway of apoptosis. It functions as a central component of the apoptosome, a multiprotein complex that activates caspase-9 in response to cellular stress, DNA damage, or developmental cues. APAF1 contains a caspase recruitment domain (CARD), which facilitates interactions with procaspase-9. and a nucleotide-binding domain that regulates its oligomerization upon binding to cytochrome c released from mitochondria. Dysregulation of APAF1 has been implicated in various diseases, including cancer (where its downregulation may contribute to resistance to apoptosis) and neurodegenerative disorders (linked to excessive apoptosis).
APAF1 antibodies are essential tools for studying apoptosis mechanisms, protein expression, and localization in cells or tissues. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies typically target specific epitopes within APAF1’s functional domains, enabling researchers to investigate its activation status, interactions with binding partners (e.g., cytochrome c, caspase-9), or alterations in disease models. Both monoclonal and polyclonal APAF1 antibodies are available, with validation often including knockout cell line controls to ensure specificity. Understanding APAF1 dynamics through antibody-based assays aids in exploring therapeutic strategies targeting apoptotic pathways.
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