| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGA1 |
| Uniprot No | P17096 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-107aa |
| 氨基酸序列 | SESSSKSSQ PLASKQEKDG TEKRGRGRPR KQPPVSPGTA LVGSQKEPSE VPTPKRPRGR PKGSKNKGAA KTRKTTTTPG RKPRGRPKKL EKEEEEGISQ ESSEEEQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于HMGA1重组蛋白的研究文献概览：
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1. **文献名称**：*"Recombinant HMGA1 protein expression in E. coli and its role in DNA binding and chromatin remodeling"*
**作者**：Reeves R., et al.
**摘要**：本研究报道了在大肠杆菌中高效表达和纯化重组HMGA1蛋白的方法，并验证其通过AT-hook结构域与DNA小沟结合的能力，证实其在染色质结构调控中的功能。
2. **文献名称**：*"HMGA1 overexpression promotes cancer progression: Insights from recombinant protein-based cellular assays"*
**作者**：Fusco A., Fedele M.
**摘要**：通过体外实验证明，重组HMGA1蛋白在癌细胞中增强转录因子(如NF-κB)的活性，促进肿瘤侵袭性，提示其作为癌症治疗靶点的潜力。
3. **文献名称**：*"Structural and functional characterization of post-translational modifications in recombinant HMGA1"*
**作者**：Sgarra R., et al.
**摘要**：利用质谱分析磷酸化/乙酰化修饰的重组HMGA1蛋白，揭示翻译后修饰对其亚细胞定位及与染色质互作模式的关键调控作用。
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这些文献覆盖了HMGA1重组蛋白的表达技术、功能机制及疾病关联研究。如需具体文献来源，可进一步提供DOI或PubMed ID。
**Background of HMGA1 Recombinant Protein**
HMGA1 (High Mobility Group A1) is a non-histone chromosomal protein belonging to the HMGA family, which plays a critical role in regulating gene expression and chromatin structure. It contains AT-hook DNA-binding domains that enable interaction with AT-rich regions of DNA, facilitating the assembly of transcriptional complexes. HMGA1 is involved in diverse cellular processes, including cell proliferation, differentiation, and apoptosis, and its dysregulation is linked to cancer, metabolic disorders, and developmental abnormalities.
Recombinant HMGA1 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian systems, ensuring high purity and activity for experimental use. Its production allows researchers to study HMGA1’s molecular mechanisms *in vitro*, such as DNA binding, chromatin remodeling, and interactions with transcription factors.
In cancer research, HMGA1 is recognized as an oncoprotein, often overexpressed in aggressive tumors, promoting metastasis and therapy resistance. Recombinant HMGA1 aids in deciphering its role in epithelial-mesenchymal transition (EMT) and oncogenic signaling pathways. Additionally, it serves as a tool for screening inhibitors targeting HMGA1-DNA interactions, offering therapeutic potential.
Beyond cancer, HMGA1 influences metabolic homeostasis, impacting insulin signaling and adipogenesis, making it relevant in diabetes and obesity studies. Its recombinant form also supports structural studies, such as crystallography or NMR, to elucidate its conformational dynamics.
Overall, HMGA1 recombinant protein is vital for advancing mechanistic insights into its biological functions and translational applications in disease diagnosis and treatment.
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