| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | IL7R; ILRA; IL7RA; CDW127; IL-7R-alpha |
| Entrez GeneID | 3575 |
| clone | 3F5D9 |
| WB Predicted band size | 51.6kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD127 (AA: extra 21-239) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD127抗体的3篇代表性文献的简要信息，涵盖基础研究及临床应用方向：
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1. **文献名称**: *CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ Treg cells*
**作者**: Seddiki, N. et al.
**摘要**: 研究发现CD127(IL-7Rα)在人类调节性T细胞(Treg)中低表达，而效应T细胞高表达。通过CD127抗体结合其他标记(如CD25)可有效区分Treg与活化效应T细胞，为免疫疾病的细胞分选提供新方法。
2. **文献名称**: *IL-7 receptor signaling is necessary for stage transition in adult B cell development through up-regulation of EBF*
**作者**: Miller, J.P. et al.
**摘要**: 利用CD127抗体阻断实验，证实IL-7受体信号通过调控转录因子EBF促进B细胞发育阶段转换，揭示CD127在淋巴细胞分化中的关键作用。
3. **文献名称**: *CD127+CCR5+ T cells are markers of latent HIV-1 infection*
**作者**: Liszewski, M.K. et al.
**摘要**: 通过CD127抗体检测发现，HIV潜伏感染的CD4+ T细胞高表达CD127和CCR5.提示CD127可作为抗病毒治疗中靶向清除病毒储存库的潜在标志物。
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以上文献分别涉及Treg细胞分选、淋巴细胞发育机制及HIV潜伏感染研究，展示了CD127抗体在基础机制探索和临床诊断中的应用价值。如需具体期刊信息或年份，可进一步补充数据库检索结果。
CD127 antibody targets the interleukin-7 receptor alpha chain (IL-7Rα), a critical component of the IL-7 receptor complex (IL-7R), which pairs with the common gamma chain (γc) to mediate signaling. CD127 is expressed on immune cells, particularly naïve and memory T cells, regulatory T cells (Tregs), and innate lymphoid cells (ILCs). It plays a vital role in lymphocyte development, homeostasis, and immune responses by binding IL-7 and thymic stromal lymphopoietin (TSLP).
CD127 antibodies are widely used in research to identify and isolate T cell subsets. For example, CD127 is co-stained with markers like CD25 to distinguish activated effector T cells (CD25+CD127+) from immunosuppressive Tregs (CD25+CD127−/low). In flow cytometry, anti-CD127 helps characterize immune dysregulation in conditions like autoimmune diseases, infections, or cancers. Therapeutic applications are under exploration, as modulating IL-7/CD127 signaling may influence T cell survival or exhaustion. Some studies target this pathway to enhance antitumor immunity or mitigate autoimmunity.
Antibody clones vary in specificity (e.g., human: A019D5. mouse: SB/199). Validation ensures minimal cross-reactivity with related receptors. Challenges include distinguishing membrane-bound vs. soluble CD127 isoforms and addressing expression heterogeneity across cell types. Recent work also links CD127 polymorphisms to disease susceptibility, underscoring its clinical relevance.
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