| WB | 1/500 - 1/2000 | Human,Rat,Monkey |
| IF | 咨询技术 | Human,Rat,Monkey |
| IHC | 1/200 - 1/1000 | Human,Rat,Monkey |
| ICC | 1/200 - 1/1000 | Human,Rat,Monkey |
| FCM | 1/200 - 1/400 | Human,Rat,Monkey |
| Elisa | 1/10000 | Human,Rat,Monkey |
| Aliases | PEPCK; PEPCK2; PEPCK-M |
| Entrez GeneID | 5106 |
| clone | 3D3D9 |
| WB Predicted band size | 70.7kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat,Monkey |
| Immunogen | Purified recombinant fragment of human PCK2 (AA: 44-175) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于PCK2(磷酸烯醇丙酮酸羧激酶2)抗体的3篇示例文献(内容为假设性概括，供参考)：
1. **文献名称**：*PCK2 regulates mitochondrial metabolism and promotes cancer cell survival under hypoxia*
**作者**：Li, X., et al.
**摘要**：本研究利用PCK2特异性抗体，通过免疫印迹和免疫荧光技术验证了PCK2在线粒体中的表达，并揭示其在缺氧条件下通过调控糖异生途径维持肿瘤细胞能量代谢的作用。
2. **文献名称**：*A novel PCK2 antibody reveals its role in hepatocellular carcinoma progression*
**作者**：Wang, Y., et al.
**摘要**：作者开发了一种高特异性PCK2单克隆抗体，并通过免疫组化分析证明PCK2在肝癌组织中高表达，其水平与患者预后不良相关，提示PCK2可能作为潜在治疗靶点。
3. **文献名称**：*PCK2-mediated metabolic reprogramming drives chemoresistance in colorectal cancer*
**作者**：Zhang, H., et al.
**摘要**：通过PCK2抗体检测结直肠癌样本，发现PCK2通过激活谷氨酰胺代谢途径促进化疗耐药，敲低PCK2可增强癌细胞对5-FU的敏感性。
注：以上为示例性内容，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
The PCK2 antibody targets phosphoenolpyruvate carboxykinase 2 (PCK2), a mitochondrial enzyme encoded by the PCK2 gene. PCK2 catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP), a rate-limiting step in gluconeogenesis and a critical node connecting the tricarboxylic acid (TCA) cycle with glucose metabolism. Unlike its cytosolic isoform PCK1. PCK2 is primarily involved in cataplerosis, replenishing TCA intermediates and regulating energy homeostasis under nutrient-deprived conditions. It plays a role in insulin secretion, amino acid metabolism, and lipid synthesis, linking mitochondrial function to cellular metabolic flexibility.
PCK2 is upregulated in various cancers, where it supports tumor growth by fueling biosynthetic pathways and mitigating oxidative stress. Its expression is regulated by hormones (e.g., glucocorticoids), dietary states, and hypoxia-inducible factors. The PCK2 antibody is widely used to study tissue-specific expression, subcellular localization, and dynamic regulation of PCK2 in metabolic disorders (e.g., diabetes, NAFLD) and cancer. Applications include Western blotting, immunohistochemistry, and immunofluorescence to explore PCK2's role in metabolic reprogramming, mitochondrial bioenergetics, and therapeutic targeting. Research using PCK2 antibodies has highlighted its dual role in both physiological adaptation and pathological processes, making it a biomarker of interest in metabolic and oncological studies.
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