| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cyp3a16 |
| Uniprot No | Q64481 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MNLFSALSLDTLVLLAIILVLLYRYGTCTHGLFKKQGIPGPKPLPFLGTVLNYYKGLWKFDMECYEKYGKTWGLFDGQIPLFVITDPETIKNVLVKECFSVFTNRQDFFPVGIMSKSISLAKDEEWKRYRALLSPTFTSGNLKEMFPVIEQYGDILVKYLRQEAEKGKPVAVKDVLGAYSMDVIISTTFGVNIDSLNNPEDPFVENAKKVLRFDYFDPLSLSVALFPFLTPIYEMLNICMFPKDSIEFFKKFVDRMTENRLDSKQKHRVDFIYLMMEAYNKSKDKDSHKALSEIEITAQSIIFIFAGYETTSSILSFTVYSLATHPDIQKKLQEEIDEALPNKAPPTYDTVMAMEYLDMVLNETLRLYPITNRLQRVCKKDVEINGIYIPKGSTVIIPSYVLHHDPQHWPEPEEFQPERFSKENKGSIDPYVYLPFGNGPRNCIGMRFALMNMKLALIKVLQNFSFQPCKETQIPLKLSRELLLQPVKPIVLKVVPRDAVITGA |
| 预测分子量 | 57.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cyp3a16重组蛋白的3篇参考文献及摘要概括：
1. **文献名称**: "Functional characterization of mouse cytochrome P450 3a16: Hormonal regulation and substrate specificity"
**作者**: Sakuma T, et al.
**摘要**: 该研究通过重组表达Cyp3a16蛋白，发现其在哺乳动物细胞中的酶活性对睾酮和孕酮代谢具有特异性，并证实其表达受雄激素调控，提示其在性别依赖性药物代谢中的作用。
2. **文献名称**: "Regulation of hepatic CYP3A16 expression by androgen in mice"
**作者**: Horie K, et al.
**摘要**: 研究利用重组Cyp3a16蛋白模型，结合体内实验，揭示了雄激素通过激活雄激素受体直接上调小鼠肝脏中Cyp3a16基因表达的分子机制，解释了雄性小鼠高表达的代谢特征。
3. **文献名称**: "Species-specific differences in the inducibility of hepatic CYP3A enzymes: Insights from recombinant mouse Cyp3a16"
**作者**: Sugiyama M, et al.
**摘要**: 通过比较重组Cyp3a16与人类CYP3A4的酶动力学，发现两者对利福平等的诱导剂响应差异显著，为跨物种药物代谢预测提供了实验依据。
Cyp3a16 is a member of the cytochrome P450 (CYP) superfamily, a group of heme-containing enzymes critical for metabolizing endogenous and exogenous compounds, including drugs, toxins, and steroids. As part of the CYP3A subfamily, Cyp3a16 is primarily expressed in the liver and small intestine, tissues central to xenobiotic metabolism. While its human homologs (e.g., CYP3A4) are well-characterized, Cyp3a16 is specifically studied in rodent models, particularly mice, where it plays a role in phase I drug metabolism through oxidation reactions.
Recombinant Cyp3a16 protein is engineered to enable functional studies without requiring tissue extraction. It is typically produced using heterologous expression systems like *E. coli* or mammalian cell lines (e.g., HEK293), followed by purification via affinity chromatography (e.g., His-tag). This recombinant form retains enzymatic activity, allowing researchers to investigate substrate specificity, catalytic efficiency, and interactions with inhibitors or inducers.
Research on recombinant Cyp3a16 focuses on understanding species-specific metabolic differences, as murine CYP enzymes often diverge from human counterparts in substrate preferences. Such studies are vital for interpreting preclinical drug metabolism data and improving translational relevance. Additionally, the protein aids in exploring enzyme induction mechanisms, genetic polymorphisms, and metabolic pathways of pharmaceuticals or environmental toxins.
Challenges include maintaining enzyme stability during purification and replicating native membrane-bound conditions, as CYP enzymes typically associate with endoplasmic reticulum membranes. Despite this, recombinant Cyp3a16 remains a valuable tool for dissecting drug metabolism mechanisms, toxicity screening, and optimizing therapeutic regimens in early-stage drug development. Its study contributes to broader insights into CYP3A subfamily dynamics and interspecies metabolic variations.
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