| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SFTA2 |
| Uniprot No | Q6UW10 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-78aa |
| 氨基酸序列 | TGPGMTLQLKLKESFLTNSSYESSFLELLEKLCLLLHLPSGTSVTLHHARSQHHVVCNT |
| 预测分子量 | 13.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SFTA2重组蛋白的3条参考文献示例(注：部分信息为虚构整合，仅作格式参考，实际文献需通过学术数据库验证)：
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1. **文献名称**：Recombinant expression and functional characterization of SFTA2 in pulmonary surfactant homeostasis
**作者**：Chen L, et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组SFTA2蛋白，证实其通过调节磷脂代谢参与肺表面活性物质的稳定性，为肺部疾病治疗提供潜在靶点。
2. **文献名称**：Structural analysis of SFTA2 reveals its role in innate immune response
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析了重组SFTA2的三维结构，发现其与病原体结合域相互作用，增强肺泡巨噬细胞的抗菌活性，提示其在宿主防御中的功能。
3. **文献名称**：SFTA2 recombinant protein alleviates acute lung injury in murine models
**作者**：Zhang R, et al.
**摘要**：动物实验表明，外源性重组SFTA2蛋白通过抑制炎症因子释放和减少肺泡上皮细胞凋亡，显著改善脂多糖诱导的急性肺损伤。
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建议通过PubMed或Web of Science检索“SFTA2 recombinant protein”获取真实文献。
**Background of SFTA2 Recombinant Protein**
SFTA2 (Surfactant-associated protein A2), also known as SP-A2. is a member of the pulmonary surfactant protein family, primarily synthesized and secreted by alveolar type II cells in the lungs. It plays a critical role in maintaining pulmonary surfactant homeostasis, reducing alveolar surface tension, and facilitating innate immune defense. Structurally, SFTA2 belongs to the collectin family, characterized by a conserved collagen-like domain at the N-terminus and a carbohydrate-recognition domain (CRD) at the C-terminus, enabling interactions with pathogens and immune cells.
As a key component of the lung’s first-line defense, SFTA2 binds to microbial surfaces (e.g., bacteria, viruses, fungi) via its CRD, promoting opsonization, phagocytosis, and clearance by alveolar macrophages. It also modulates inflammatory responses by regulating cytokine production and immune cell activation. Genetic polymorphisms or deficiencies in SFTA2 have been linked to increased susceptibility to respiratory infections, chronic lung diseases, and neonatal respiratory distress syndrome.
Recombinant SFTA2 protein is engineered using heterologous expression systems (e.g., mammalian, bacterial, or insect cells) to study its structural and functional properties. Its applications span basic research—investigating surfactant biology, host-pathogen interactions, and immune regulation—and therapeutic development, such as designing surfactant replacement therapies or immunomodulatory agents for lung injuries. Challenges in production include preserving post-translational modifications (e.g., glycosylation) critical for its bioactivity.
Overall, SFTA2 recombinant protein serves as a vital tool for dissecting pulmonary immunity and exploring novel treatments for respiratory disorders.
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