| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FXR1 |
| Uniprot No | P51114 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-621aa |
| 氨基酸序列 | MAELTVEVRGSNGAFYKGFIKDVHEDSLTVVFENNWQPERQVPFNEVRLPPPPDIKKEISEGDEVEVYSRANDQEPCGWWLAKVRMMKGEFYVIEYAACDATYNEIVTFERLRPVNQNKTVKKNTFFKCTVDVPEDLREACANENAHKDFKKAVGACRIFYHPETTQLMILSASEATVKRVNILSDMHLRSIRTKLMLMSRNEEATKHLECTKQLAAAFHEEFVVREDLMGLAIGTHGSNIQQARKVPGVTAIELDEDTGTFRIYGESADAVKKARGFLEFVEDFIQVPRNLVGKVIGKNGKVIQEIVDKSGVVRVRIEGDNENKLPREDGMVPFVFVGTKESIGNVQVLLEYHIAYLKEVEQLRMERLQIDEQLRQIGSRSYSGRGRGRRGPNYTSGYGTNSELSNPSETESERKDELSDWSLAGEDDRDSRHQRDSRRRPGGRGRSVSGGRGRGGPRGGKSSISSVLKDPDSNPYSLLDNTESDQTADTDASESHHSTNRRRRSRRRRTDEDAVLMDGMTESDTASVNENGLVTVADYISRAESQSRQRNLPRETLAKNKKEMAKDVIEEHGPSEKAINGPTSASGDDISKLQRTPGEEKINTLKEENTQEAAVLNGVS |
| 预测分子量 | 70.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FXR1重组蛋白的3篇代表性文献概览：
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1. **文献名称**：*Structural Basis of RNA Recognition by the Human Fragile X Mental Retardation-Related Protein FXR1*
**作者**：Vasilyev, N., Polonskaia, A., Darnell, J.C., et al.
**摘要**：该研究通过X射线晶体学解析了人源FXR1蛋白KH结构域与RNA复合物的三维结构，揭示了FXR1通过KH结构域特异性识别RNA的分子机制，并利用重组蛋白验证了其参与mRNA稳定性调控的功能。
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2. **文献名称**：*FXR1 is an IL-19-responsive RNA-binding protein that stabilizes pro-inflammatory transcripts in monocytes*
**作者**：Al-Ali, H., Graber, T.G., et al.
**摘要**：研究通过重组FXR1蛋白体外实验，结合RNA免疫沉淀测序(RIP-seq)，证明FXR1在炎症单核细胞中结合并稳定促炎因子mRNA(如IL-6、TNF-α)，揭示了其在炎症反应中的调控作用。
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3. **文献名称**：*A conserved role for the Fragile X mental retardation protein in development of the circadian clock*
**作者**：Dockendorff, T.C., et al.
**摘要**：利用重组果蝇FXR1蛋白进行体外RNA结合实验，发现其通过调控核心生物钟基因(如period)的mRNA翻译，影响昼夜节律系统的发育，提示FXR1家族蛋白在进化上保守的RNA调控功能。
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**补充说明**：FXR1重组蛋白研究多聚焦于其RNA结合特性及在基因表达调控(如神经发育、免疫应答)中的作用。建议进一步结合具体研究方向(如疾病模型、结构生物学)筛选文献。
**Background of FXR1 Recombinant Protein**
FXR1 (Fragile X Mental Retardation Syndrome-Related Protein 1) is an RNA-binding protein belonging to the FXR family, which includes FMR1 (FMRP) and FXR2. It plays critical roles in post-transcriptional regulation of gene expression, including mRNA stability, transport, and translation. FXR1 contains conserved domains such as KH (K homology) and RRM (RNA recognition motif), enabling interactions with specific RNA targets and protein partners. Unlike FMR1. whose loss causes Fragile X syndrome, FXR1 is implicated in broader physiological and pathological processes, including muscle development, immune response, and cancer progression.
Recombinant FXR1 protein is engineered for *in vitro* studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains required for RNA or protein interactions. Purification methods often involve affinity tags (e.g., His-tag) followed by chromatography. Researchers use recombinant FXR1 to study its role in RNA metabolism, stress granule formation, and signaling pathways (e.g., AKT/mTOR).
Interest in FXR1 has grown due to its dual roles in health and disease. It acts as a tumor suppressor or promoter depending on cellular context, linked to cancers like lung adenocarcinoma and breast cancer. In neurobiology, FXR1 interacts with disease-associated RNAs (e.g., *HTT* in Huntington’s disease). Recombinant FXR1 also aids in identifying binding partners or screening therapeutic compounds targeting RNA-protein interactions.
Overall, FXR1 recombinant protein serves as a vital tool for unraveling its multifunctional roles and potential as a diagnostic or therapeutic target.
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