| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | MLP; SMUC; MUC-2 |
| Entrez GeneID | 4583 |
| clone | 1C8A5 |
| WB Predicted band size | 540kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human MUC2 (AA: 4373-4557) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于MUC2抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *"Production and characterization of a new monoclonal antibody against the C-terminal domain of human MUC2"*
**作者**: Bara J, et al.
**摘要**: 该研究报道了一种针对人MUC2蛋白C端结构域的新型单克隆抗体的开发，验证了其在免疫组化(IHC)和Western blot中的特异性，并证明其适用于检测结直肠癌和正常肠道组织中的MUC2表达。
2. **文献名称**: *"MUC2 mucin expression in inflammatory bowel disease and colorectal cancer: insights from a novel antibody-based detection method"*
**作者**: Johansson MEV, et al.
**摘要**: 研究利用特异性MUC2抗体分析IBD(炎症性肠病)和结直肠癌中MUC2黏液层的改变，发现MUC2表达缺失与肠道屏障功能受损及肿瘤进展相关，强调了该抗体在病理研究中的实用性。
3. **文献名称**: *"Comparative analysis of MUC2 antibodies for the study of goblet cell differentiation in murine models"*
**作者**: Bergstrom KSB, et al.
**摘要**: 研究比较了多种MUC2抗体在小鼠肠杯状细胞分化模型中的应用效果，确定了特定抗体(如CLH2克隆)在免疫荧光和流式细胞术中的高敏感性和特异性，为黏液分泌研究提供工具支持。
(注：以上文献信息为示例，实际引用时请核对期刊及作者准确性。)
The MUC2 antibody is a crucial tool for studying mucin 2 (MUC2), a high-molecular-weight glycoprotein central to forming the protective mucus layer in the gastrointestinal and respiratory tracts. MUC2. encoded by the *MUC2* gene on chromosome 11p15.5. is the primary secretory mucin produced by intestinal goblet cells. It plays a vital role in maintaining mucosal barrier integrity, lubrication, and pathogen defense by creating a gel-like matrix. Dysregulation of MUC2 expression or glycosylation is linked to inflammatory bowel disease (IBD), colorectal cancer, and chronic respiratory conditions.
MUC2 antibodies, often targeting its variable tandem repeat (VTR) or glycosylated regions, are widely used in research and diagnostics. Polyclonal and monoclonal antibodies (e.g., clones 4F1. CCP58) enable detection via Western blot, immunohistochemistry (IHC), or ELISA, helping assess mucus composition and goblet cell function. Some antibodies distinguish between glycoforms, aiding studies on aberrant glycosylation in cancer. Notably, MUC2 antibodies must account for structural complexity—its heavily O-glycosylated, cysteine-rich domains require specific epitope recognition. Commercial antibodies vary in specificity, necessitating validation for experimental contexts. Recent applications include exploring gut microbiota interactions, drug delivery systems exploiting mucus properties, and biomarker development for mucin-secreting tumors.
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