| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200-1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200-1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LSN; SPN; GALGP; GPL115 |
| Entrez GeneID | 6693 |
| clone | 3E12F3 |
| WB Predicted band size | 40.3kda |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human CD43(AA: extra(20-169)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于CD43抗体的3篇代表性文献(虚构示例，供参考)：
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1. **标题**: *CD43: A multifunctional glycoprotein in T-cell activation and adhesion*
**作者**: Smith A, et al.
**摘要**: 研究揭示了CD43在T细胞活化中的双重作用，其胞外段通过调节细胞间黏附影响免疫突触形成，而胞内段与细胞骨架信号通路相互作用。抗CD43抗体可阻断T细胞过度活化，为自身免疫疾病治疗提供新靶点。
2. **标题**: *CD43 expression in acute myeloid leukemia: Prognostic implications and therapeutic targeting*
**作者**: Lee JH, et al.
**摘要**: 通过分析AML患者样本，发现CD43高表达与不良预后相关。体外实验表明，抗CD43抗体偶联药物可特异性诱导白血病细胞凋亡，提示其作为靶向治疗的潜力。
3. **标题**: *Structural characterization of CD43-specific monoclonal antibodies for flow cytometry standardization*
**作者**: Garcia-Romo S, et al.
**摘要**: 系统比较了5种商用抗CD43抗体的表位识别特性，建立基于克隆DF-T1的标准化流式检测方案，显著提高淋巴细胞亚群分析的重复性，为临床免疫分型提供可靠工具。
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注：以上为模拟内容，实际文献需通过PubMed或学术数据库检索。如需真实文献，建议使用关键词“CD43 antibody function/therapy/diagnosis”查询近年高被引论文。
CD43. also known as leukosialin or sialophorin, is a transmembrane glycoprotein highly expressed on leukocytes, particularly T cells, neutrophils, and certain B cell subsets. Its extracellular domain contains numerous O-linked glycosylation sites, contributing to its role in cell adhesion, migration, and immune regulation. CD43 antibodies target specific epitopes of this protein and are widely used in research and diagnostics to identify immune cell populations, study activation states, and investigate pathologies like leukemia or autoimmune disorders.
First characterized in the 1980s, CD43 antibodies (e.g., clones like DF-T1 or L60) became crucial tools for flow cytometry and immunohistochemistry. Different clones recognize distinct glycosylation-dependent or peptide epitopes, influencing their applications. For instance, some detect CD43 only on activated T cells or specific leukemic blasts. In disease contexts, CD43 expression patterns help differentiate malignancies (e.g., T-ALL vs. B-cell lymphomas) and correlate with autoimmune conditions like lupus, where altered CD43-mediated signaling may impair immune tolerance.
Functionally, CD43 antibodies have revealed the protein's dual roles: its large, negatively charged extracellular domain can both inhibit cell-cell interactions (e.g., T cell-APC binding) and promote recruitment to inflammatory sites via interactions with receptors like ICAM-1. Recent studies also explore CD43's involvement in HIV infection and checkpoint modulation, highlighting its therapeutic potential. Despite structural complexities due to heavy glycosylation, CD43 remains a valuable biomarker and research target in immunology and hematology.
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