| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEACAM5 |
| Uniprot No | P06731 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 35-685aa |
| 氨基酸序列 | KLTIESTPFNVAEGKEVLLLVHNLPQHLFGYSWYKGERVDGNRQIIGYVIGTQQATPGPAYSGREIIYPNASLLIQNIIQNDTGFYTLHVIKSDLVNEEATGQFRVYPELPKPSISSNNSKPVEDKDAVAFTCEPETQDATYLWWVNNQSLPVSPRLQLSNGNRTLTLFNVTRNDTASYKCETQNPVSARRSDSVILNVLYGPDAPTISPLNTSYRSGENLNLSCHAASNPPAQYSWFVNGTFQQSTQELFIPNITVNNSGSYTCQAHNSDTGLNRTTVTTITVYAEPPKPFITSNNSNPVEDEDAVALTCEPEIQNTTYLWWVNNQSLPVSPRLQLSNDNRTLTLLSVTRNDVGPYECGIQNKLSVDHSDPVILNVLYGPDDPTISPSYTYYRPGVNLSLSCHAASNPPAQYSWLIDGNIQQHTQELFISNITEKNSGLYTCQANNSASGHSRTTVKTITVSAELPKPSISSNNSKPVEDKDAVAFTCEPEAQNTTYLWWVNGQSLPVSPRLQLSNGNRTLTLFNVTRNDARAYVCGIQNSVSANRSDPVTLDVLYGPDTPIISPPDSSYLSGANLNLSCHSASNPSPQYSWRINGIPQQHTQVLFIAKITPNNNGTYACFVSNLATGRNNSIVKSITVSASGTSPGLSA |
| 预测分子量 | 100.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEACAM5重组蛋白的模拟参考文献示例(文献为虚构，仅供格式参考)：
1. **文献名称**: "Expression and Purification of Recombinant CEACAM5 for Immunoassay Development"
**作者**: Smith A, et al.
**摘要**: 本研究描述了一种在大肠杆菌中高效表达和纯化重组CEACAM5蛋白的方法，验证其抗原性可用于开发高灵敏度的ELISA检测试剂盒，适用于结直肠癌患者血清标志物分析。
2. **文献名称**: "Structural Characterization of CEACAM5 Glycoforms Using Recombinant Protein Technology"
**作者**: Zhang L, et al.
**摘要**: 通过哺乳动物细胞表达系统获得不同糖基化修饰的重组CEACAM5蛋白，结合质谱和X射线晶体学分析其结构，揭示了糖基化对CEACAM5与抗体结合能力的影响。
3. **文献名称**: "Targeted Drug Delivery via CEACAM5-Specific Antibody-Conjugated Nanoparticles Loaded with Recombinant CEACAM5"
**作者**: Kim J, et al.
**摘要**: 开发了一种基于重组CEACAM5蛋白的纳米药物递送系统，通过偶联CEACAM5特异性抗体，实现靶向肿瘤细胞的高效药物释放，并在小鼠模型中显示抗肿瘤活性。
4. **文献名称**: "Role of Recombinant CEACAM5 in Modulating Immune Checkpoint Responses in Pancreatic Cancer"
**作者**: Garcia R, et al.
**摘要**: 研究重组CEACAM5蛋白对胰腺癌微环境中T细胞活化的调控作用，发现其通过结合TIM-3受体抑制免疫应答，为联合免疫治疗提供新靶点。
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注：以上文献为模拟内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。
**Background of CEACAM5 Recombinant Protein**
CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5), also known as CD66e, is a glycosylated cell surface protein belonging to the CEACAM family, which plays roles in intercellular adhesion, immune modulation, and intracellular signaling. It is encoded by the *CEACAM5* gene and is structurally characterized by an N-terminal immunoglobulin-like domain, followed by multiple immunoglobulin constant-like domains, and a glycosylphosphatidylinositol (GPI) anchor for membrane attachment. CEACAM5 is normally expressed in mucosal tissues but is markedly overexpressed in various carcinomas, including colorectal, gastric, pancreatic, and lung cancers, making it a well-established tumor biomarker for diagnosis and monitoring.
Recombinant CEACAM5 protein is produced using genetic engineering techniques, typically by expressing the gene in prokaryotic (e.g., *E. coli*) or eukaryotic (e.g., mammalian or insect) cell systems. This allows large-scale production of purified, biologically active protein for research and clinical applications. The recombinant form retains key epitopes and structural features of the native protein, enabling its use in functional studies to investigate CEACAM5’s role in tumor progression, metastasis, and immune evasion. For instance, it aids in exploring interactions with CEACAM family members or immune receptors like TIM-3.
In diagnostics, recombinant CEACAM5 serves as a critical component in immunoassays (e.g., ELISA) to quantify CEACAM5 levels in patient sera, aiding cancer detection and prognosis. Therapeutically, it is utilized to develop and validate targeted therapies, such as monoclonal antibodies or CAR-T cells, that specifically bind CEACAM5 on cancer cells. Additionally, it supports vaccine development by providing antigenic material to induce immune responses against CEACAM5-expressing tumors.
Overall, CEACAM5 recombinant protein is a vital tool bridging basic research and clinical innovation in oncology.
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