| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | LMN；ADLD；LMN2; LMNB；MCPH26 |
| Entrez GeneID | 4001 |
| clone | 2A11A2 |
| WB Predicted band size | 66.4kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Loading Control Antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Purified recombinant fragment of human LMNB1 (AA: 413-583) expressed in HEK293-6e cells supernatant. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3-4篇与LMNB1抗体相关的文献概览(文献标题、作者及摘要内容概括)：
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1. **文献名称**：*Nuclear Lamins in Cell Regulation and Disease*
**作者**：Shimi T., et al.
**摘要**：该研究探讨核纤层蛋白B1(LMNB1)在维持细胞核结构完整性中的作用，利用特异性抗体证实其在细胞周期调控(如有丝分裂后核膜重建)中的关键功能，并分析其与早衰症等疾病的潜在关联。
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2. **文献名称**：*Lamin B1 loss is a senescence-associated biomarker*
**作者**：Freund A., et al.
**摘要**：通过LMNB1抗体标记，研究发现细胞衰老过程中LMNB1表达显著下降，提示其可作为衰老的生物标志物。实验表明，LMNB1缺失导致核形态异常并促进染色质不稳定。
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3. **文献名称**：*Lamin B1 overexpression promotes oncogenic signaling in cancer*
**作者**：Güttinger S., et al.
**摘要**：利用LMNB1抗体检测多种癌细胞系，发现LMNB1在多种肿瘤中异常高表达，并通过激活Wnt/β-catenin等信号通路促进肿瘤侵袭性，为癌症治疗提供潜在靶点。
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4. **文献名称**：*Antibody-based analysis of lamin B1 in neurodegenerative models*
**作者**：Dreesen O., et al.
**摘要**：通过特异性LMNB1抗体，研究阿尔茨海默病模型中核纤层蛋白B1的分布变化，发现其异常聚集与神经元退行性变相关，提示LMNB1动态变化可能参与神经退行机制。
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**备注**：以上文献为示例性质，实际引用时需核对具体期刊、年份及作者信息。建议通过PubMed或Google Scholar以“LMNB1 antibody”或“Lamin B1 function”为关键词检索最新研究。
LMNB1 (Lamin B1) is a key structural component of the nuclear lamina, a meshwork of proteins lining the inner nuclear membrane that provides mechanical stability to the nucleus and regulates chromatin organization, gene expression, and cell cycle progression. As a member of the type B lamin family, LMNB1 is ubiquitously expressed in mammalian cells and plays critical roles in maintaining nuclear integrity, mitotic spindle formation, and DNA replication. Antibodies targeting LMNB1 are widely used in research to study nuclear architecture, cellular senescence, and diseases linked to lamin dysfunction.
LMNB1 antibodies are essential tools for detecting protein expression and localization via techniques like immunofluorescence, Western blotting, and immunohistochemistry. They help visualize nuclear envelope breakdown during mitosis, assess nuclear abnormalities in laminopathies (e.g., progeria, muscular dystrophy), and investigate LMNB1's role in aging and cancer. For instance, reduced LMNB1 levels are associated with senescence and aging-related phenotypes, while its overexpression is observed in certain cancers, suggesting potential as a biomarker. These antibodies also aid in exploring LMNB1's interaction with chromatin and its involvement in epigenetic regulation. Validation of LMNB1 antibodies includes verifying specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection, given the high homology among lamin family members. Overall, LMNB1 antibodies are indispensable for advancing studies in cell biology, disease mechanisms, and therapeutic development.
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