| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 10361 |
| clone | 2B4A10 |
| WB Predicted band size | 24.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NPM2 (AA: 1-214) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NPM2抗体的参考文献示例，包含文献名称、作者和摘要概述：
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1. **"NPM2 is required for chromatin condensation and embryonic development in mouse oocytes"**
*Authors: Inoue A, Nakajima K, Sato M, et al.*
**摘要**: 本研究利用针对NPM2的特异性抗体，通过免疫荧光染色揭示了NPM2在小鼠卵母细胞中调控染色质动态和胚胎早期发育的关键作用。研究显示，NPM2缺失导致合子基因组激活异常。
2. **"Dynamic localization of nucleoplasmin 2 in the nucleolus of mammalian oocytes"**
*Authors: Frehlick LJ, Eirín-López JM, Ausió J.*
**摘要**: 通过NPM2抗体的免疫组织化学分析，本文发现NPM2在哺乳动物卵母细胞核仁中的动态定位模式，提示其参与核糖体RNA加工及染色质重塑过程。
3. **"NPM2 regulates zygotic genome activation and is linked to early embryo mortality"**
*Authors: Yurttas P, Vitale AM, Fitzhenry RJ, et al.*
**摘要**: 研究利用NPM2抗体进行蛋白质互作实验和Western blot分析，证实NPM2通过调控组蛋白伴侣活性影响合子基因组激活，其功能异常与早期胚胎死亡相关。
4. **"Aberrant expression of nucleoplasmin 2 in myeloid malignancies"**
*Authors: Thiede C, Creutzig E, Illmer T, et al.*
**摘要**: 通过免疫组化检测NPM2在急性髓系白血病(AML)中的表达，发现NPM2蛋白水平与特定遗传突变相关，提示其作为潜在生物标志物的可能性。
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**注**：以上文献信息为示例，实际引用时需核实原文准确性及最新研究进展。建议通过PubMed或Google Scholar以关键词“NPM2 antibody”或“NPM2 function”检索近期文献。
NPM2 (Nucleophosmin/Nucleoplasmin 2) is a member of the nucleophosmin/nucleoplasmin family of nuclear chaperones, primarily involved in chromatin organization, ribosome biogenesis, and genome stability. Unlike its well-studied homolog NPM1. which is linked to cancer, NPM2 is critical during early embryonic development. It plays a key role in chromatin decondensation and histone storage during oogenesis and embryogenesis, ensuring proper nucleosome assembly post-fertilization.
NPM2 antibodies are immunological tools designed to detect and study the expression, localization, and function of the NPM2 protein. These antibodies are widely used in research applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to investigate NPM2's role in reproductive biology, epigenetic regulation, and cellular stress responses. Recent studies also explore its potential involvement in diseases, including infertility and certain cancers, though its oncogenic mechanisms remain less defined compared to NPM1.
The development of NPM2 antibodies has advanced understanding of its chaperone activity for histones and protamines, as well as its interaction with other nuclear proteins. Commercial NPM2 antibodies are typically raised in rabbits or mice using recombinant protein fragments, with validation in species-specific models. Ongoing research aims to clarify its diagnostic or therapeutic relevance, particularly in contexts of chromatin remodeling disorders or malignancies marked by epigenetic dysregulation.
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