| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Slc17a6 |
| Uniprot No | Q9P2U8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-582aa |
| 氨基酸序列 | MESVKQRILAPGKEGLKNFAGKSLGQIYRVLEKKQDTGETIELTEDGKPLEVPERKAPLCDCTCFGLPRRYIIAIMSGLGFCISFGIRCNLGVAIVDMVNNSTIHRGGKVIKEKAKFNWDPETVGMIHGSFFWGYIITQIPGGYIASRLAANRVFGAAILLTSTLNMLIPSAARVHYGCVIFVRILQGLVEGVTYPACHGIWSKWAPPLERSRLATTSFCGSYAGAVIAMPLAGILVQYTGWSSVFYVYGSFGMVWYMFWLLVSYESPAKHPTITDEERRYIEESIGESANLLGAMEKFKTPWRKFFTSMPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFEISKVGMLSAVPHLVMTIIVPIGGQIADFLRSKQILSTTTVRKIMNCGGFGMEATLLLVVGYSHTRGVAISFLVLAVGFSGFAISGFNVNHLDIAPRYASILMGISNGVGTLSGMVCPIIVGAMTKNKSREEWQYVFLIAALVHYGGVIFYAIFASGEKQPWADPEETSEEKCGFIHEDELDEETGDITQNYINYGTTKSYGATTQANGGWPSGWEKKEEFVQGEVQDSHSYKDRVDYS |
| 预测分子量 | 64.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Slc17a6(VGLUT2)重组蛋白的3篇参考文献示例：
1. **文献名称**：*"Structure and function of the vesicular glutamate transporter VGLUT2"*
**作者**：Eriksen J. et al.
**摘要**：通过重组蛋白表达及冷冻电镜技术解析了VGLUT2的跨膜结构，揭示了其谷氨酸转运的分子机制及pH依赖性调控特征。
2. **文献名称**：*"Reconstitution of vesicular glutamate transport using purified VGLUT2 in proteoliposomes"*
**作者**：Herring B.E., Ueda T.
**摘要**：利用重组Slc17a6蛋白重构脂质体系统，验证其谷氨酸转运活性及氯离子通道功能的耦合机制。
3. **文献名称**：*"Phosphorylation-dependent regulation of VGLUT2 trafficking by recombinant protein analysis"*
**作者**：Charbonneau B.R. et al.
**摘要**：通过重组蛋白突变实验，证明Slc17a6的胞内段磷酸化修饰影响其突触囊泡定位及神经递质释放效率。
(注：以上文献信息为模拟示例，实际文献需通过数据库检索确认。)
Slc17a6. also known as vesicular glutamate transporter 2 (VGluT2), is a member of the solute carrier 17 (SLC17) family of transmembrane transport proteins. It plays a critical role in the central nervous system by packaging the excitatory neurotransmitter glutamate into synaptic vesicles, enabling its release during neurotransmission. VGluT2 is predominantly expressed in glutamatergic neurons, particularly in subcortical regions, the brainstem, and thalamocortical pathways, distinguishing it from the closely related VGluT1. which is more abundant in cortical areas.
The Slc17a6 gene encodes a 12-transmembrane domain protein that relies on a proton gradient to transport glutamate into vesicles. Dysregulation of VGluT2 has been implicated in neurological disorders such as epilepsy, Parkinson’s disease, and schizophrenia, making it a target for neuropharmacological research. Recombinant Slc17a6 protein is produced through genetic engineering, typically by expressing the cloned gene in heterologous systems like mammalian cells, bacteria, or insect cells. This allows researchers to study its structure, transport mechanisms, and interactions with potential modulators in controlled environments.
Recombinant VGluT2 is essential for in vitro assays, drug screening, and structural studies (e.g., cryo-EM or X-ray crystallography) to elucidate its conformational dynamics. Its applications extend to developing therapies targeting glutamatergic signaling imbalances, a hallmark of numerous psychiatric and neurodegenerative conditions. Ongoing research also explores its role in synaptic plasticity and neural circuit regulation, highlighting its broader impact on cognitive and sensory processing. The availability of recombinant Slc17a6 protein thus serves as a cornerstone for advancing both basic neuroscience and translational medical studies.
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