| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT2 |
| Uniprot No | P35908 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MSCQISCKSRGRGGGGGGFRGFSSGSAVVSGGSRRSTSSFSCLSRHGGGGGGFGGGGFGSRSLVGLGGTKSISISVAGGGGGFGAAGGFGGRGGGFGGGSSFGGGSGFSGGGFGGGGFGGGRFGGFGGPGGVGGLGGPGGFGPGGYPGGIHEVSVNQSLLQPLNVKVDPEIQNVKAQEREQIKTLNNKFASFIDKVRFLEQQNQVLQTKWELLQQMNVGTRPINLEPIFQGYIDSLKRYLDGLTAERTSQNSELNNMQDLVEDYKKKYEDEINKRTAAENDFVTLKKDVDNAYMIKVELQSKVDLLNQEIEFLKVLYDAEISQIHQSVTDTNVILSMDNSRNLDLDSIIAEVKAQYEEIAQRSKEEAEALYHSKYEELQVTVGRHGDSLKEIKIEISELNRVIQRLQGEIAHVKKQCKNVQDAIADAEQRGEHALKDARNKLNDLEEALQQAKEDLARLLRDYQELMNVKLALDVEIATYRKLLEGEECRMSGDLSSNVTVSVTSSTISSNVASKAAFGGSGGRGSSSGGGYSSGSSSYGSGGRQSGSRGGSGGGGSISGGGYGSGGGSGGRYGSGGGSKGGSISGGGYGSGGGKHSSGGGSRGGSSSGGGYGSGGGGSSSVKGSSGEAFGSSVTFSFR |
| 预测分子量 | 72.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT2重组蛋白的3篇示例性参考文献(内容为虚构，仅用于格式参考)：
1. **《Expression and Purification of Recombinant Human Keratin 2 (KRT2) in E. coli》**
- **作者**: Smith J, et al.
- **摘要**: 本研究通过大肠杆菌表达系统成功克隆并纯化了重组人KRT2蛋白，优化了表达条件以提高溶解度，并通过质谱验证其结构完整性，为后续功能研究奠定基础。
2. **《KRT2 Recombinant Protein Modulates Epidermal Differentiation in vitro》**
- **作者**: Lee S, Kim M.
- **摘要**: 利用哺乳动物细胞表达的重组KRT2蛋白，发现其通过调控角化细胞分化相关信号通路(如Notch通路)，影响表皮屏障功能，提示KRT2在皮肤疾病中的潜在作用。
3. **《Structural Analysis of Recombinant KRT2 Filaments by Cryo-Electron Microscopy》**
- **作者**: Zhang R, et al.
- **摘要**: 通过冷冻电镜解析了重组KRT2蛋白的纤维组装结构，揭示了其与其他角蛋白(如KRT1)的相互作用界面，为理解角蛋白网络稳定性机制提供分子依据。
(注：以上文献为模拟内容，实际文献需通过PubMed或Google Scholar检索关键词如"recombinant KRT2 protein"获取。)
Keratin 2 (KRT2), a member of the type II keratin family, is a structural protein critical for maintaining epidermal integrity. Encoded by the *KRT2* gene, it is primarily expressed in the suprabasal layers of stratified epithelia, particularly the skin’s epidermis. KRT2 pairs with type I keratins (e.g., KRT10) to form intermediate filaments, providing mechanical resilience to epithelial cells and regulating differentiation processes. Its expression is tightly linked to epidermal maturation, making it a biomarker for keratinocyte differentiation.
Dysregulation of KRT2 is implicated in skin disorders like psoriasis and ichthyosis. In psoriasis, reduced KRT2 levels correlate with hyperproliferation and impaired differentiation of keratinocytes. Conversely, mutations in *KRT2* are associated with ichthyosis bullosa of Siemens, a genetic disorder characterized by skin fragility and scaling. These clinical connections highlight KRT2’s role in skin homeostasis and disease pathogenesis.
Recombinant KRT2 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties and interactions. The recombinant form retains functional domains, enabling research on filament assembly, protein-protein interactions, and signaling pathways in vitro. Purification techniques like affinity chromatography ensure high purity, essential for structural studies (e.g., X-ray crystallography) or functional assays. Researchers also use recombinant KRT2 to develop disease models, screen therapeutic compounds, and investigate epidermal barrier mechanisms. Its application extends to antibody production for diagnostic tools targeting keratin-related disorders. Overall, recombinant KRT2 serves as a vital tool for unraveling skin biology and advancing translational dermatology research.
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