| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT32 |
| Uniprot No | Q14532 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-448aa |
| 氨基酸序列 | MTSSCCVTNNLQASLKSCPRPASVCSSGVNCRPELCLGYVCQPMACLPSVCLPTTFRPASCLSKTYLSSSCQAASGISGSMGPGSWYSEGAFNGNEKETMQFLNDRLASYLTRVRQLEQENAELESRIQEASHSQVLTMTPDYQSHFRTIEELQQKILCTKAENARMVVNIDNAKLAADDFRAKYEAELAMRQLVEADINGLRRILDDLTLCKADLEAQVESLKEELMCLKKNHEEEVGSLRCQLGDRLNIEVDAAPPVDLTRVLEEMRCQYEAMVEANRRDVEEWFNMQMEELNQQVATSSEQLQNYQSDIIDLRRTVNTLEIELQAQHSLRDSLENTLTESEARYSSQLAQMQCMITNVEAQLAEIRADLERQNQEYQVLLDVRARLEGEINTYRSLLENEDCKLPCNPCSTPSCTTCVPSPCVPRTVCVPRTVGMPCSPCPQGRY |
| 预测分子量 | 57.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT32重组蛋白的3篇模拟参考文献及其摘要概括：
1. **《重组人KRT32蛋白在大肠杆菌中的高效表达与纯化》**
*作者：Langbein L, Schweizer J*
摘要：本研究成功构建了KRT32基因的重组表达载体，利用大肠杆菌系统实现高效表达，并通过亲和层析技术纯化获得高纯度蛋白。该重组蛋白可用于后续抗体开发及角蛋白功能研究。
2. **《KRT32重组蛋白在毛囊角质化过程中的作用机制》**
*作者：Rogers GE, Yu J*
摘要：通过体外实验证实，重组KRT32蛋白与KRT83等角蛋白相互作用，参与毛囊角质细胞骨架网络的形成。研究揭示了KRT32在维持毛发机械强度中的关键功能。
3. **《KRT32基因突变体的重组表达与病理相关性分析》**
*作者：Shimomura Y, Smith FJD*
摘要：针对遗传性皮肤病患者的KRT32突变位点，构建了重组突变体蛋白。体外实验表明，特定突变导致蛋白稳定性下降，为相关疾病的分子机制提供了实验依据。
(注：上述文献为基于领域内常见研究方向的模拟内容，实际文献需通过学术数据库检索确认。)
Keratins are a diverse family of structural proteins that form intermediate filaments in epithelial cells, playing critical roles in maintaining cellular integrity, mechanical stability, and stress resistance. KRT32 (Keratin 32) is a type II acidic keratin primarily expressed in specialized epithelial tissues, including hair follicles, nail beds, and certain stratified squamous epithelia. It is encoded by the *KRT32* gene and pairs with specific type I keratins to form heterodimeric complexes essential for cytoskeletal organization. Dysregulation or mutations in KRT32 have been linked to hair and nail disorders, such as monilethrix and pachyonychia congenita-like phenotypes, highlighting its biological importance.
Recombinant KRT32 protein is produced using biotechnological platforms (e.g., bacterial, insect, or mammalian expression systems) to enable functional studies and therapeutic exploration. By cloning the *KRT32* gene into expression vectors, researchers can generate purified protein for investigating its structural properties, interaction partners, and role in epithelial differentiation. Recombinant versions also serve as antigens for antibody development or tools to model keratin-related pathologies in vitro. Recent studies suggest KRT32 may influence hair shaft formation and matrix cell adhesion, making it a potential target for treating alopecia or genetic keratinopathies. Its recombinant form bridges basic research and translational applications, offering insights into epithelial biology and precision medicine strategies.
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