| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cacng1 |
| Uniprot No | Q06432 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-222aa |
| 氨基酸序列 | MSQTKMLKVRVTLFCILAGIVLAMTAVVTDHWAVLSPHMEHHNTTCEAAHFGLWRICTKRIPMDDSKTCGPITLPGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAIAIFSLGFIILGSLCVLLSLGKKRDYLLRPASMFYAFAGLCILVSVEVMRQSVKRMIDSEDTVWIEYYYSWSFACACAAFILLFLGGLALLLFSLPRMPRNPWESCMDAEPEH |
| 预测分子量 | 31.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cacng1重组蛋白的3篇代表性文献摘要整理：
1. **《Cacng1 regulates postsynaptic AMPA receptor trafficking》**
- 作者：Chen L et al.
- 摘要：研究揭示了Cacng1重组蛋白通过调控突触后膜AMPA受体的内吞和再循环，影响神经突触可塑性。实验通过体外表达重组Cacng1并利用神经元模型，证明其与AMPA受体亚基的直接相互作用可调节兴奋性突触传递。
2. **《Structural and functional analysis of the calcium channel γ1 subunit》**
- 作者：Kang MG et al.
- 摘要：通过原核系统表达并纯化Cacng1重组蛋白，结合X射线晶体学解析其三维结构。发现其跨膜结构域对钙通道的电压敏感性和失活过程具有调控作用，为癫痫相关突变研究提供结构基础。
3. **《Cacng1 modulates seizure susceptibility in mice via NMDA receptor regulation》**
- 作者：Wang Y et al.
- 摘要：利用重组Cacng1蛋白进行体外结合实验，发现其与NMDA受体NR2B亚基存在物理互作。动物实验表明Cacng1敲除小鼠癫痫阈值显著降低，提示该蛋白可能通过稳定突触NMDA受体复合物影响癫痫发生机制。
注：以上为模拟文献摘要，实际研究中建议通过PubMed/Google Scholar检索最新论文(关键词：Cacng1 recombinant protein, AMPA receptor, ion channel structure)。如需真实文献，可提供具体研究方向进一步筛选。
**Background of Cacng1 Recombinant Protein**
Cacng1 (Calcium Channel Gamma Subunit 1), a member of the transmembrane AMPA receptor regulatory protein (TARP) family, is a critical auxiliary subunit of voltage-gated calcium channels (VGCCs) and AMPA-type glutamate receptors. It plays a regulatory role in ion channel trafficking, localization, and functional modulation, influencing neuronal excitability and synaptic transmission. The Cacng1 gene encodes a protein with four transmembrane domains, contributing to the structural stability of calcium channels and modulating their electrophysiological properties.
Recombinant Cacng1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical interactions, structural features, and mechanistic roles in vitro. Its production typically involves cloning the Cacng1 cDNA into expression vectors, followed by purification via affinity tags (e.g., His-tag). This recombinant tool enables researchers to explore Cacng1’s involvement in neurological processes, such as synaptic plasticity, and its association with disorders like epilepsy, neuropathic pain, and psychiatric conditions.
Studies using Cacng1 recombinant protein have highlighted its role in regulating calcium influx and receptor desensitization, which are pivotal for understanding neuronal signaling cascades. Additionally, it serves as a valuable reagent for antibody development, protein-protein interaction assays, and drug discovery targeting ion channelopathies. Despite its established importance, the precise molecular mechanisms and tissue-specific functions of Cacng1 remain under investigation, emphasizing the need for further structural and functional studies. Overall, recombinant Cacng1 provides a versatile platform for dissecting its contributions to both normal physiology and disease pathogenesis.
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