| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMN |
| Uniprot No | P51645 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-357aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSVSKLWVPNTDFDVAANWSQNRTPCAGG AVEFPADKMVSVLVQEGHAVSDMLLPLDGELVLASGAGFGVSDVGSHLDC GAGEPAVFRDSDRFSWHDPHLWRSGDEAPGLFFVDAERVPCRHDDVFFPP SASFRVGLGPGASPVRVRSISALGRTFTRDEDLAVFLASRAGRLRFHGPG ALSVGPEDCADPSGCVCGNAEAQPWICAALLQPLGGRCPQAACHSALRPQ GQCCDLCGAVVLLTHGPAFDLERYRARILDTFLGLPQYHGLQVAVSKVPR SSRLREADTEIQVVLVENGPETGGAGRLARALLADVAENGEALGVLEATM RESGAHVWGSS |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMN重组蛋白的3篇参考文献示例(注：文献信息为模拟示例，实际引用时请核实原文)：
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1. **标题**: "Functional characterization of recombinant AMN protein in vitamin B12 uptake"
**作者**: Smith J, et al.
**摘要**: 研究通过哺乳动物细胞系统表达重组AMN蛋白，证实其与cubilin蛋白协同介导肠道维生素B12内吞，为Imerslund-Gräsbeck综合征的机制提供实验依据。
2. **标题**: "Recombinant AMN extracellular domain production and structural analysis"
**作者**: Zhang Y, et al.
**摘要**: 利用昆虫细胞体系成功表达并纯化AMN蛋白胞外结构域，通过X射线晶体学解析其三维结构，揭示其与配体结合的潜在位点。
3. **标题**: "AMN-cubilin complex stabilization by recombinant AMN in renal proximal tubule cells"
**作者**: Köhler A, et al.
**摘要**: 发现重组AMN蛋白可通过稳定cubilin-megalin复合物增强肾脏细胞的内吞功能，提示其在治疗蛋白尿相关肾病中的潜在应用价值。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“AMN recombinant protein”“Amnionless expression”或结合疾病关键词(如“Imerslund-Gräsbeck syndrome”)。
**Background of Recombinant AMN Protein**
The recombinant AMN (Amnionless) protein is a biologically engineered form of the human AMN protein, a critical component of the cubam receptor complex. This receptor, formed by the interaction of AMN with cubilin, plays a vital role in vitamin B12 (cobalamin) absorption in the ileum and protein reabsorption in the kidney. AMN ensures the proper cellular localization and stabilization of cubilin, facilitating its function in nutrient uptake. Mutations in the *AMN* gene are linked to Imerslund-Gräsbeck syndrome (IGS), a rare autosomal recessive disorder characterized by vitamin B12 deficiency and proteinuria.
Recombinant AMN protein is typically produced using expression systems such as bacterial (e.g., *E. coli*), mammalian, or insect cell cultures. These systems enable large-scale production of the protein with high purity, retaining its functional properties. The recombinant form is instrumental in studying the molecular mechanisms of cubam-mediated nutrient uptake, disease modeling, and drug discovery. It also holds therapeutic potential, particularly in developing protein replacement therapies for AMN-deficient IGS patients or gene therapy approaches to restore functional AMN expression.
Additionally, recombinant AMN serves as a critical tool in structural biology, aiding in the characterization of cubilin-AMN interactions and the design of targeted therapies. Its applications extend to diagnostic assays for vitamin B12-related disorders and biomarker research. By bridging basic science and clinical innovation, recombinant AMN protein continues to advance our understanding of metabolic and renal pathologies while offering promising avenues for therapeutic intervention.
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