| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT14 |
| Uniprot No | P02533 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-472aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MTTCSRQFTS SSSMKGSCGI GGGIGGGSSR ISSVLAGGSC RAPSTYGGGL SVSSSRFSSG GAYGLGGGYG GGFSSSSSSF GSGFGGGYGG GLGTGLGGGF GGGFAGGDGL LVGSEKVTMQ NLNDRLASYL DKVRALEEAN ADLEVKIRDW YQRQRPAEIK DYSPYFKTIE DLRNKILTAT VDNANVLLQI DNARLAADDF RTKYETELNL RMSVEADING LRRVLDELTL ARADLEMQIE SLKEELAYLK KNHEEEMNAL RGQVGGDVNV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEEWFFTKT EELNREVATN SELVQSGKSE ISELRRTMQN LEIELQSQLS MKASLENSLE ETKGRYCMQL AQIQEMIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLSSSQ FSSGSQSSRD VTSSSRQIRT KVMDVHDGKV VSTHEQVLRT KN |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT14重组蛋白的模拟参考文献示例(实际文献需通过学术数据库检索确认)：
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1. **标题**: *"Recombinant Keratin 14 Protein Expression and Its Role in Epidermal Cell Adhesion"*
**作者**: Smith J, et al.
**摘要**: 研究报道了通过大肠杆菌系统高效表达重组KRT14蛋白的方法，并验证其通过调控细胞间连接蛋白(如β-catenin)影响上皮细胞粘附功能的机制。
2. **标题**: *"KRT14 Recombinant Protein as a Biomarker in Cutaneous Squamous Cell Carcinoma"*
**作者**: Lee H, et al.
**摘要**: 利用哺乳动物细胞表达系统制备高纯度KRT14重组蛋白，发现其在皮肤鳞状细胞癌患者血清中异常表达，可能作为早期诊断标志物。
3. **标题**: *"Functional Characterization of Recombinant KRT14 Mutants in Epidermolysis Bullosa Simplex"*
**作者**: Zhang R, et al.
**摘要**: 通过昆虫细胞杆状病毒系统表达多种KRT14突变体重组蛋白，揭示其导致表皮松解症(EBS)的分子机制，为基因治疗提供靶点。
4. **标题**: *"High-Throughput Purification of Recombinant KRT14 for Drug Screening Applications"*
**作者**: Kumar S, et al.
**摘要**: 开发了一种基于亲和层析的快速纯化重组KRT14蛋白的技术，应用于针对角蛋白相关遗传病的小分子药物筛选平台。
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**说明**：以上为模拟文献，建议通过PubMed、Web of Science等平台检索真实文献(关键词：KRT14 recombinant protein, expression, purification, application)。实际研究多涉及KRT14在皮肤疾病、癌症及干细胞分化中的功能探索。
**Background of KRT14 Recombinant Protein**
Keratin 14 (KRT14) is a type I intermediate filament protein encoded by the *KRT14* gene, primarily expressed in the basal layer of stratified epithelial tissues. It pairs with keratin 5 (KRT5) to form a heterodimeric complex, which assembles into a cytoskeletal network crucial for maintaining mechanical integrity, cell shape, and stress resilience in epithelial cells.
Mutations in *KRT14* are linked to genetic disorders such as epidermolysis bullosa simplex (EBS), a blistering skin condition caused by fragile keratinocytes prone to rupture under minor mechanical stress. Studying KRT14’s structure-function relationships is vital for understanding disease mechanisms and developing targeted therapies.
Recombinant KRT14 protein is produced using expression systems (e.g., *E. coli*, mammalian cells) to generate purified, biologically active forms of the protein. This engineered protein retains critical domains, including the central α-helical rod domain essential for filament assembly and the head/tail regions involved in interactions with other keratins and cellular components.
Applications of recombinant KRT14 span *in vitro* disease modeling, drug screening for EBS, and testing gene therapy vectors. It also serves as an antigen for antibody development in diagnostic assays. Furthermore, studies leveraging recombinant KRT14 have advanced insights into epithelial cell biology, wound healing, and cancer metastasis, as aberrant KRT14 expression is observed in certain carcinomas.
Overall, KRT14 recombinant protein is a key tool for bridging molecular research with clinical applications in dermatology and oncology.
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