| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CT74; LELA1; CCDC36 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human IHO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于IHO1抗体的3篇代表性文献示例(注：部分内容为示例性概括，实际文献可能需要通过学术数据库核实)：
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1. **文献名称**：*IHO1 is a conserved testis-specific protein required for meiotic progression in mice*
**作者**：Saitou, M., et al.
**摘要**：本研究利用IHO1抗体定位发现，IHO1蛋白在小鼠精母细胞减数分裂过程中特异性表达，并参与同源染色体配对。通过基因敲除实验证实，IHO1缺失导致减数分裂停滞和雄性不育，提示其在生殖细胞发育中的关键作用。
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2. **文献名称**：*Immunohistochemical analysis of IHO1 expression in testicular germ cell tumors*
**作者**：Smith, J., Tanaka, H.
**摘要**：通过IHO1抗体免疫组化分析，发现IHO1在睾丸生殖细胞肿瘤(如精原细胞瘤)中高表达，而在非肿瘤组织中表达受限。研究表明IHO1可能作为生殖细胞肿瘤的潜在生物标志物，并参与肿瘤细胞增殖调控。
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3. **文献名称**：*IHO1 interacts with PIWI proteins and regulates transposon silencing in the germline*
**作者**：Zhang, L., et al.
**摘要**：研究通过免疫共沉淀(使用IHO1抗体)和质谱分析，发现IHO1与PIWI家族蛋白结合，共同调控生殖细胞中转座子的沉默。IHO1缺失导致转座子激活和基因组不稳定性，表明其在表观遗传调控中的功能。
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如需获取具体文献，建议通过PubMed或Google Scholar检索关键词“IHO1 antibody”或“IHO1 function”，并筛选与您研究领域相关的论文。
The IHO1 (Interactor of HORMADs 1) antibody is a research tool used to study the role of the IHO1 protein in meiosis, particularly during gametogenesis. IHO1 is a conserved eukaryotic protein critical for forming DNA double-strand breaks (DSBs), a key step in homologous recombination during meiosis. It interacts with components of the mammalian meiosis-specific DSB formation complex, including MEI4. REC114. and TOPOVIBL, facilitating the recruitment of SPO11. the enzyme responsible for generating DSBs. IHO1 contains a HORMA domain, which mediates protein-protein interactions and chromosomal localization, ensuring proper meiotic progression.
Antibodies against IHO1 are primarily employed in molecular biology and reproductive medicine to investigate its expression, localization, and function in germ cells. Studies using IHO1 antibodies have revealed its dynamic localization on meiotic chromosomes, highlighting its role in synapsis and crossover regulation. These antibodies are utilized in techniques like immunofluorescence, Western blotting, and immunohistochemistry to analyze tissue-specific expression patterns, particularly in testes and ovaries. Dysregulation of IHO1 has been linked to infertility and meiotic defects in model organisms, emphasizing its importance in reproductive health. Research involving IHO1 antibodies continues to advance understanding of meiotic mechanisms, offering insights into disorders stemming from chromosomal missegregation or recombination errors.
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