| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | OBR; OB-R; CD295; LEP-R; LEPRD |
| WB Predicted band size | 132 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human LEPR |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LEPR抗体的3-4篇虚构参考文献示例(基于学术文献常见研究方向，非真实文献)：
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1. **文献名称**: "Monoclonal Antibodies Targeting LEPR Reveal Its Role in Obesity-Related Metabolic Dysregulation"
**作者**: Smith A, et al. (2015)
**摘要**: 研究通过开发特异性LEPR单克隆抗体，揭示了瘦素受体在肥胖小鼠模型中信号传导异常的具体机制，发现抗体阻断LEPR可加剧胰岛素抵抗，提示其在代谢调控中的关键作用。
2. **文献名称**: "Structural Characterization of LEPR Extracellular Domain Using Cryo-EM and Antibody Mapping"
**作者**: Zhang L, et al. (2018)
**摘要**: 利用冷冻电镜技术和抗体验证，解析了LEPR胞外区的高分辨率结构，并鉴定出抗体结合表位，为开发靶向LEPR的抗体药物提供结构生物学基础。
3. **文献名称**: "LEPR-Specific Antibody Therapy Attenuates Breast Cancer Progression in Preclinical Models"
**作者**: Johnson R, et al. (2020)
**摘要**: 研究发现LEPR在乳腺癌细胞中高表达，通过抗LEPR抗体阻断受体活性可抑制肿瘤生长和转移，提示其作为癌症免疫治疗潜在靶点的可能性。
4. **文献名称**: "Autoantibodies Against LEPR in Autoimmune Hypothalamic Dysfunction Syndromes"
**作者**: Tanaka K, et al. (2022)
**摘要**: 首次报道了自身免疫性疾病患者体内存在抗LEPR自身抗体，这些抗体通过干扰瘦素信号导致下丘脑功能障碍，可能解释部分代谢综合征患者的病理机制。
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(注：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台检索真实文献。)
**Background of LEPR Antibodies**
The leptin receptor (LEPR), a key player in energy homeostasis, mediates the effects of leptin—a hormone secreted by adipose tissue that regulates appetite, metabolism, and body weight. LEPR belongs to the class I cytokine receptor family and exists in multiple splice variants, with the long isoform (LEPRb) being critical for intracellular signaling via JAK-STAT pathways. Dysregulation of leptin-LEPR signaling is linked to obesity, metabolic disorders, and immune dysfunction.
LEPR antibodies are essential tools for studying receptor expression, localization, and function. They enable detection of LEPR in tissues (e.g., hypothalamus, immune cells) using techniques like immunohistochemistry, Western blotting, or flow cytometry. Specific antibodies can distinguish between LEPR isoforms or phosphorylated forms, aiding research into signaling mechanisms. Additionally, neutralizing LEPR antibodies help explore leptin resistance, a hallmark of obesity, while agonist antibodies may probe therapeutic strategies.
Research applications span obesity, diabetes, and cancer, as leptin influences tumor growth and immune responses. Mutations in *LEPR* cause severe early-onset obesity, underscoring its physiological importance. Commercial LEPR antibodies vary in specificity (monoclonal vs. polyclonal) and cross-reactivity across species, requiring validation for experimental models. Overall, LEPR antibodies remain vital for unraveling metabolic regulation and developing targeted therapies.
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