| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | IMD9; TAM2; ORAT1; CRACM1; TMEM142A |
| WB Predicted band size | 33 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ORAI1(L2) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于ORAI1(L2)抗体的代表性文献，简要总结如下：
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1. **文献名称**：*ORAI1 mutations and immunodeficiencies: Insights into calcium signaling in lymphocyte function*
**作者**：Feske, S. et al.
**摘要**：该研究利用ORAI1特异性抗体(克隆L2)检测了免疫缺陷患者的淋巴细胞中ORAI1蛋白表达水平，发现某些突变导致ORAI1功能缺失，证实其在钙离子介导的T细胞活化中的关键作用。
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2. **文献名称**：*Structural basis of ORAI1 channel activation by STIM1*
**作者**：Hou, X. et al.
**摘要**：通过ORAI1(L2)抗体的免疫共沉淀实验，揭示了STIM1与ORAI1的相互作用机制，阐明了钙池操纵性钙内流(SOCE)的分子结构基础，为靶向治疗提供了依据。
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3. **文献名称**：*Targeting ORAI1 in cancer: A novel therapeutic strategy*
**作者**：Chen, Y.F. et al.
**摘要**：研究使用ORAI1(L2)抗体分析多种肿瘤细胞系中的蛋白表达，发现ORAI1高表达与癌细胞增殖和转移相关，抑制其活性可减少肿瘤生长，提示其作为癌症治疗靶点的潜力。
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**备注**：文献中提到的“L2”可能指特定抗体克隆编号(如Santa Cruz Biotechnology的Anti-ORAI1 clone L2)，需根据实验背景确认。建议通过PubMed或Web of Science以“ORAI1 antibody L2”为关键词进一步筛选具体研究。
The ORAI1 protein, a key component of the calcium release-activated calcium (CRAC) channel, plays a critical role in store-operated calcium entry (SOCE), a process essential for immune cell activation, gene transcription, and cellular homeostasis. ORAI1 interacts with stromal interaction molecule 1 (STIM1), which senses endoplasmic reticulum (ER) calcium depletion and activates ORAI1 to mediate extracellular calcium influx. Dysregulation of ORAI1 is linked to immune disorders, including severe combined immunodeficiency (SCID) and autoimmune diseases.
The ORAI1(L2) antibody specifically targets an extracellular epitope on the second loop (L2) of the ORAI1 protein. This region is critical for channel gating and STIM1-ORAI1 interaction, making the antibody a valuable tool for studying CRAC channel structure-function relationships. It is widely used in techniques like immunofluorescence, flow cytometry, and Western blot to detect ORAI1 expression in immune cells (e.g., T cells, mast cells) and other tissues. Additionally, ORAI1(L2) antibodies have been employed in functional studies to block CRAC channel activity, helping elucidate its role in calcium signaling pathways, immune responses, and diseases such as cancer or inflammation. Researchers also utilize these antibodies to explore therapeutic strategies targeting ORAI1 in autoimmune conditions or immunodeficiencies, underscoring their importance in both basic and translational research.
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