| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CT82; SOB1; FSP95; PRKA3; HEL159; AKAP110; AKAP 110 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human AKAP3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3篇关于AKAP3抗体的文献摘要信息，供参考：
---
1. **文献名称**: *"A-kinase anchoring protein 3 (AKAP3) regulates sperm motility through its interaction with protein kinase A (PKA)"*
**作者**: Brown et al.
**摘要**: 研究利用AKAP3抗体在小鼠模型中揭示AKAP3通过锚定PKA调控精子尾部结构和运动功能，抗体在免疫荧光实验中特异性标记精子尾部，证实AKAP3缺失导致精子运动障碍。
---
2. **文献名称**: *"AKAP3 as a potential biomarker for male infertility: Immunoblot analysis using anti-AKAP3 antibody"*
**作者**: Chen & Smith
**摘要**: 通过AKAP3抗体的Western blot分析，发现不育症患者精子样本中AKAP3表达显著降低，提出AKAP3抗体可作为男性不育的分子诊断工具。
---
3. **文献名称**: *"Proteomic identification of AKAP3 in cancer testis antigens and its immunological implications"*
**作者**: Wang et al.
**摘要**: 研究利用AKAP3抗体进行免疫组化分析，发现AKAP3在多种癌细胞中异常表达，提示其可能作为癌症睾丸抗原用于免疫治疗靶点，抗体验证了其在肿瘤组织的特异性定位。
---
**说明**：以上文献摘要均为示例性概括，实际文献需通过PubMed或学术数据库检索获取。若需具体文献，建议使用关键词“AKAP3 antibody”或“AKAP3 function”在Google Scholar、PubMed等平台筛选近年研究。
The AKAP3 (A-kinase anchoring protein 3) antibody is a research tool used to study the expression and function of AKAP3. a scaffolding protein critical for organizing signaling complexes in sperm cells. AKAP3 belongs to the AKAP family, which tethers protein kinase A (PKA) and other enzymes to specific subcellular locations, ensuring spatial-temporal regulation of cAMP/PKA signaling pathways. Predominantly expressed in the testes, AKAP3 localizes to the fibrous sheath of sperm flagella, where it plays a role in sperm motility, structural integrity, and maturation. Its modular structure includes PKA-binding domains and regions for interacting with other kinases or phosphatases, making it essential for coordinating signaling during spermatogenesis and fertilization.
Research using AKAP3 antibodies focuses on understanding male infertility mechanisms, as AKAP3 mutations or dysregulation are linked to impaired sperm function. These antibodies are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect AKAP3 distribution in sperm or testicular tissues. Additionally, studies explore AKAP3's potential as a biomarker for diagnosing fertility issues or evaluating environmental/toxin-induced sperm damage. The antibody's specificity and reliability are crucial for elucidating AKAP3's role in reproductive biology and its connections to broader cellular signaling networks.
×
