| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Y4; PP1; PPYR1; NPY4-R |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NPY4R |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NPY4R抗体的参考文献及其摘要概述：
1. **文献名称**：*"Development and characterization of a specific NPY4 receptor antibody for immunohistochemical studies"*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种针对NPY4R的高特异性多克隆抗体，并通过免疫印迹和免疫组化验证其灵敏度。结果显示该抗体能有效检测小鼠脑组织和肠道中的NPY4R蛋白分布，为研究其在摄食调控中的作用提供了工具。
2. **文献名称**：*"NPY4 receptor expression in the hypothalamus: Antibody validation and functional implications"*
**作者**：Brown K, et al.
**摘要**：通过对比多种抗体，本研究筛选出适用于人脑组织NPY4R检测的商业化抗体，并利用基因敲除模型验证其特异性。发现NPY4R在下丘脑中高表达，可能通过调节能量代谢影响肥胖发生。
3. **文献名称**：*"Localization of NPY4 receptors in the murine gastrointestinal tract using a novel monoclonal antibody"*
**作者**：Lee J, et al.
**摘要**：开发了一种新型NPY4R单克隆抗体，用于研究受体在小鼠胃肠道中的定位。结果显示NPY4R在肠神经系统中广泛表达，提示其可能参与肠道运动及分泌功能的调控。
4. **文献名称**：*"Antibody-based detection of NPY4R in adipose tissue: Association with metabolic syndrome"*
**作者**：Garcia R, et al.
**摘要**：利用特异性抗体分析肥胖人群中NPY4R在脂肪组织的表达变化，发现其与胰岛素抵抗相关，提示NPY4R可能成为代谢综合征的潜在治疗靶点。
(注：以上文献为示例，实际引用时需核对真实存在的论文及作者信息。)
The NPY4R antibody is a research tool designed to detect and study the neuropeptide Y receptor Y4 (NPY4R), a G protein-coupled receptor (GPCR) belonging to the neuropeptide Y (NPY) receptor family. NPY4R primarily binds pancreatic polypeptide (PP), a gut-derived hormone involved in regulating appetite, energy homeostasis, and gastrointestinal functions. Structurally, NPY4R consists of seven transmembrane domains and signals through Gαi/o proteins, inhibiting cAMP production and modulating intracellular pathways like MAPK/ERK. It is expressed in peripheral tissues (e.g., pancreas, intestines) and specific brain regions, suggesting roles in both central and peripheral physiological processes.
Antibodies targeting NPY4R enable researchers to investigate its expression, localization, and function in normal and pathological states. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and flow cytometry. Studies involving NPY4R antibodies have explored its involvement in obesity, metabolic disorders, and cancer, where receptor dysregulation may contribute to disease progression. For example, NPY4R activation has been linked to appetite suppression and antitumor effects in certain cancers.
The development and validation of NPY4R antibodies are critical for advancing understanding of its signaling mechanisms and therapeutic potential. Challenges include ensuring specificity due to homology with other NPY receptors. Reliable NPY4R antibodies are essential tools for basic research and drug discovery, particularly in metabolic and oncological studies.
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