| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRP2BP |
| Uniprot No | Q9P2M1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-347aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMKLTSEKLPKNPFYASVSQYAAKNQKF FQWKKEKTDYTHANLVDKALQLLKERILKGDTLAYFLRGQLYFEEGWYEE ALEQFEEIKEKDHQATYQLGVMYYDGLGTTLDAEKGVDYMKKILDSPCPK ARHLKFAAAYNLGRAYYEGKGVKRSNEEAERLWLIAADNGNPKASVKAQS MLGLYYSTKEPKELEKAFYWHSEACGNGNLESQGALGLMYLYGQGIRQDT EAALQCLREAAERGNVYAQGNLVEYYYKMKFFTKCVAFSKRIADYDEVHD IPMIAQVTDCLPEFIGRGMAMASFYHARCLQLGLGITRDETTAKHYYSKA CRLNPALADELHSLLIRQRI |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRP2BP重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Recombinant LRP2BP modulates Wnt/β-catenin signaling in renal cell models*
**作者**: Smith J, et al.
**摘要**: 本研究通过表达并纯化重组人源LRP2BP蛋白，探究其与Wnt信号通路的相互作用。实验表明，LRP2BP通过与低密度脂蛋白受体相关蛋白2(LRP2)结合，抑制β-catenin的核转位，提示其在调节肾脏细胞分化中的潜在作用。
2. **文献名称**: *Structural characterization of LRP2BP and its role in cellular uptake*
**作者**: Chen L, et al.
**摘要**: 作者利用大肠杆菌系统成功表达重组LRP2BP，并通过X射线晶体学解析其三维结构。功能实验显示，该蛋白可增强细胞对脂蛋白颗粒的内吞作用，为LRP2BP在代谢疾病中的机制研究提供依据。
3. **文献名称**: *LRP2BP recombinant protein alleviates fibrosis in murine liver models*
**作者**: Wang Y, et al.
**摘要**: 研究利用哺乳动物细胞表达体系制备功能性LRP2BP重组蛋白，并在小鼠肝纤维化模型中验证其治疗效果。结果显示，LRP2BP通过拮抗TGF-β信号通路显著减少胶原沉积，提示其作为抗纤维化治疗药物的潜力。
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注：以上文献为示例性内容，实际研究中需根据具体论文补充。若需真实文献，建议通过PubMed或Web of Science以“LRP2BP recombinant”为关键词检索。
**Background of LRP2BP Recombinant Protein**
LRP2BP (Low-Density Lipoprotein Receptor-Related Protein 2 Binding Protein), also known as Megalin or LRP2. is a multifunctional endocytic receptor belonging to the LDL receptor family. It plays a critical role in cellular uptake of various ligands, including lipoproteins, vitamins, hormones, and signaling molecules. LRP2BP is predominantly expressed in polarized epithelial cells, particularly in the kidneys, lungs, and brain, where it facilitates receptor-mediated endocytosis and transcytosis, contributing to nutrient transport and cellular signaling.
The recombinant LRP2BP protein is engineered using advanced biotechnological methods to mimic the native protein's structure and function. It is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as glycosylation, which are essential for ligand-binding activity. The recombinant form retains key domains, including ligand-binding cysteine-rich repeats and epidermal growth factor (EGF)-like domains, enabling interaction with diverse ligands like apolipoproteins, vitamin-binding proteins, and amyloid-β.
Research involving LRP2BP recombinant protein focuses on elucidating its role in physiological and pathological processes. It is implicated in renal tubular reabsorption, vitamin homeostasis, and neurodevelopment. Dysregulation of LRP2BP is associated with diseases such as Dent disease, Alzheimer’s disease, and cancer metastasis. The recombinant protein serves as a vital tool for studying these mechanisms, screening therapeutic agents, and developing diagnostic assays.
In drug development, LRP2BP recombinant protein aids in understanding receptor-ligand interactions and designing targeted therapies. Its applications extend to biomarker discovery and regenerative medicine, highlighting its significance in both basic research and clinical innovation.
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