| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | ASMD; ASOD; PTX3; CTPP4; CTRCT11 |
| WB Predicted band size | 32 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human PITX3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PITX3抗体相关的文献摘要(虚构示例，仅供参考格式)：
1. **文献名称**: *PITX3 antibody validation in midbrain dopaminergic neurons*
**作者**: Smith A, et al.
**摘要**: 该研究通过免疫组化和Western blot验证了PITX3抗体在小鼠中脑多巴胺能神经元中的特异性，发现其在帕金森病模型中表达显著下调。
2. **文献名称**: *Role of PITX3 in ocular development: Antibody-based localization analysis*
**作者**: Chen L, et al.
**摘要**: 利用PITX3抗体进行胚胎小鼠眼组织染色，揭示PITX3在晶状体发育中的时空表达模式，并证实其突变导致先天性白内障。
3. **文献名称**: *PITX3 as a biomarker for dopaminergic progenitor cells*
**作者**: Kim S, et al.
**摘要**: 通过流式细胞术和免疫荧光，证明PITX3抗体可特异性标记人多能干细胞分化的多巴胺能前体细胞，支持其在细胞治疗研究中的应用。
(注：以上内容为模拟，实际文献需通过PubMed或Google Scholar检索关键词“PITX3 antibody”获取。)
The PITX3 antibody is a crucial tool in studying the role of the PITX3 (Paired-Like Homeodomain Transcription Factor 3) protein, a transcription factor essential for embryonic development, particularly in eye lens formation and midbrain dopaminergic neuron differentiation. PITX3. a member of the PITX homeobox gene family, regulates gene expression by binding to specific DNA sequences, influencing cellular differentiation and survival. Its dysfunction is linked to congenital eye disorders (e.g., cataracts, anterior segment dysgenesis) and neurodegenerative conditions like Parkinson’s disease due to dopaminergic neuron loss.
Researchers use PITX3 antibodies primarily in immunohistochemistry (IHC), immunofluorescence (IF), and Western blot (WB) to detect PITX3 expression patterns in tissues (e.g., lens, brain, skeletal muscle) and cell models. These antibodies, often raised in rabbits or mice, are validated for specificity through knockout controls or siRNA knockdown. Commercial variants may differ in clonality (monoclonal/polyclonal), epitope targets, and species reactivity (human, mouse, rat).
PITX3 antibodies also aid in studying developmental biology, stem cell differentiation into dopaminergic neurons, and disease mechanisms. For instance, they help assess PITX3’s role in maintaining midbrain neuron populations or its dysregulation in cataract models. Quality validation (e.g., lot consistency, cross-reactivity checks) is critical to ensure reproducibility, especially given PITX3’s structural homology with other PITX family members.
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