| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human NEK10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NEK10抗体的3篇代表性文献的简要概括(基于真实研究，具体标题和作者为模拟)：
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1. **文献名称**：*NEK10 regulates breast cancer proliferation via estrogen receptor signaling*
**作者**：Smith A, et al.
**摘要**：本研究利用NEK10特异性抗体验证了其在乳腺癌细胞中的表达，发现NEK10通过磷酸化雌激素受体(ERα)促进肿瘤细胞增殖，抗体阻断实验表明其可能成为ER阳性乳腺癌的治疗靶点。
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2. **文献名称**：*A cell cycle-dependent NEK10-DNA-PK complex regulates G2/M checkpoint*
**作者**：Chen L, et al.
**摘要**：通过NEK10抗体的免疫共沉淀(Co-IP)实验，揭示了NEK10与DNA-PK在G2/M期形成复合物，调控DNA损伤修复和细胞周期检查点，抗体染色显示其定位在DNA损伤位点。
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3. **文献名称**：*Autoinhibitory mechanism of NEK10 kinase activity revealed by phospho-specific antibodies*
**作者**：Yamamoto K, et al.
**摘要**：开发了针对NEK10自磷酸化位点的特异性抗体，证实其激酶活性受C端结构域的自抑制调控，为靶向NEK10的药物设计提供了结构依据。
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**备注**：以上文献信息为示例性质，实际研究需通过PubMed或Web of Science以“NEK10 antibody”为关键词检索近期论文。NEK10抗体相关研究多聚焦于其在癌症、DNA损伤响应及激酶调控机制中的应用。
NEK10 (NIMA-related kinase 10) is a member of the NEK serine/threonine kinase family, which plays diverse roles in cell cycle regulation, DNA damage response, and mitotic progression. Unlike other NEK members (e.g., NEK2. NEK7), NEK10 is less characterized but has gained attention for its potential involvement in cancer biology and cellular stress pathways. It contains a unique N-terminal domain and a conserved kinase domain, suggesting specialized regulatory mechanisms. Studies indicate NEK10 participates in the G2/M checkpoint control, possibly through interactions with cell cycle regulators like ATR/ATM kinases, and modulates DNA repair processes. Its role in phosphorylating downstream targets, such as components of the ERK or Akt pathways, remains under investigation.
NEK10 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess protein levels, subcellular distribution (e.g., cytoplasmic vs. nuclear), and post-translational modifications. Dysregulation of NEK10 has been implicated in breast cancer progression, with some studies linking its overexpression to tumor growth and therapy resistance. However, conflicting reports on its pro- or anti-tumorigenic roles highlight the need for further research. Commercial NEK10 antibodies vary in specificity; validation using knockout cell lines is critical due to potential cross-reactivity with other NEKs. Ongoing research aims to clarify NEK10's signaling networks and therapeutic potential in precision oncology.
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