| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | p85; ASPP1; p53BP2-like |
| WB Predicted band size | 120 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human PPP1R13B |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于PPP1R13B抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*"RAI2/ASPP1 inhibits p53-dependent apoptosis in colorectal cancer cells"*
**作者**：V. Sottile et al.
**摘要**：该研究利用针对PPP1R13B(RAI2/ASPP1)的特异性抗体，通过Western blot和免疫荧光技术，揭示了其在结直肠癌细胞中抑制p53依赖性凋亡的作用，表明其可能作为肿瘤进展的潜在调控因子。
2. **文献名称**：*"Expression and clinical significance of PPP1R13B in breast cancer"*
**作者**：L. Wang et al.
**摘要**：作者使用PPP1R13B抗体进行免疫组化分析，发现其在乳腺癌组织中表达显著降低，且低表达与患者预后不良相关，提示其可能作为乳腺癌的生物标志物。
3. **文献名称**：*"PPP1R13B regulates the NF-κB signaling pathway in inflammatory responses"*
**作者**：M. Tanaka et al.
**摘要**：该研究通过免疫共沉淀(Co-IP)和Western blot，结合PPP1R13B抗体，证实该蛋白通过调控NF-κB通路参与炎症反应，为抗炎治疗提供了新靶点。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar以“PPP1R13B antibody”或“RAI2/ASPP1”为关键词检索最新研究。
PPP1R13B (Protein Phosphatase 1 Regulatory Subunit 13B), also known as iASPP (inhibitor of Apoptosis Stimulating Protein of p53), is a regulatory protein involved in apoptosis, stress response, and tumorigenesis. It functions as an inhibitor of p53 and NF-κB transcriptional activity by binding to their transactivation domains, thereby modulating cell survival and death pathways. PPP1R13B is overexpressed in several cancers, where it suppresses apoptosis and promotes chemoresistance, making it a potential therapeutic target.
Antibodies targeting PPP1R13B are essential tools for studying its expression, localization, and interactions in cellular models and clinical samples. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess PPP1R13B levels in cancer tissues, correlate its expression with disease progression, or evaluate its role in cellular stress responses. Some studies also employ PPP1R13B antibodies in co-immunoprecipitation (Co-IP) to explore its binding partners, such as p53 or PP1 phosphatase.
Research using PPP1R13B antibodies has highlighted its dual role: while it promotes survival in normal cells under stress, its dysregulation in cancer contributes to tumor aggressiveness. Recent work focuses on developing inhibitors that disrupt PPP1R13B-p53 interactions to reactivate apoptosis in malignancies. Validated antibodies are critical for these investigations, requiring specificity checks via knockout controls or siRNA knockdown to ensure accuracy in experimental outcomes.
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