| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | CX45; GJA7 |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GJC1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GJC1抗体的3篇代表性文献的简要总结(信息基于公开研究整理，部分内容为简化概括)：
1. **文献名称**：*GJC1 (Connexin45) deficiency in mice leads to heart malformation with disrupted ventricular expression of connexin43*
**作者**：Nishii K. et al.
**摘要**：本研究利用GJC1基因敲除小鼠模型，结合GJC1特异性抗体进行免疫组化和Western blot分析，发现GJC1缺失导致胚胎期心脏发育异常，并影响邻近连接蛋白Cx43的表达分布，提示其在心脏电信号传导中的关键作用。
2. **文献名称**：*Expression of connexin45 in adult mammalian central nervous system: Analysis with a novel connexin45-specific antibody*
**作者**：Maxeiner S. et al.
**摘要**：作者开发了一种高特异性GJC1(Cx45)抗体，并通过免疫荧光和免疫电镜技术验证其在成年小鼠大脑皮层和海马中的表达，揭示了Cx45在中枢神经系统突触可塑性中的潜在功能。
3. **文献名称**：*Connexin45 mediates heterocellular metabolic coupling in the diabetic retina*
**作者**：Koval M. et al.
**摘要**：通过GJC1抗体标记视网膜细胞，研究发现糖尿病模型小鼠中Cx45介导的细胞间代谢耦联异常，可能与糖尿病视网膜病变的血管渗漏和神经元损伤相关，为治疗靶点提供新思路。
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**注**：以上信息为示例性概括，实际文献需通过PubMed、Google Scholar等平台按标题或作者检索原文获取完整内容。如需具体文献DOI或发表年份，可进一步提供关键词辅助查询。
**Background of GJC1 Antibody**
GJC1 (Gap Junction Protein Gamma 1), also known as Connexin 45 (Cx45), is a transmembrane protein that forms gap junctions, specialized intercellular channels enabling direct exchange of ions, metabolites, and signaling molecules between adjacent cells. These channels play critical roles in synchronizing cellular activities, particularly in excitable tissues like the heart, nervous system, and retina. GJC1 is expressed in specific cell types, including cardiomyocytes, neurons, and oligodendrocytes, and is involved in embryonic development, electrical conduction, and tissue homeostasis.
Antibodies targeting GJC1 are essential tools for studying its expression, localization, and function in physiological and pathological contexts. They are widely used in techniques such as immunohistochemistry (IHC), Western blotting, and immunofluorescence to investigate GJC1's role in cardiac arrhythmias, neurodegenerative disorders, and cancer progression. For example, altered GJC1 expression has been linked to impaired cardiac conduction, demyelinating diseases, and tumor metastasis.
Despite their utility, GJC1 antibodies require rigorous validation due to potential cross-reactivity with other connexin family members (e.g., Cx43. Cx30) that share structural homology. Researchers prioritize antibodies with confirmed specificity to ensure accurate interpretation of data, particularly in complex tissues where multiple connexins coexist. Overall, GJC1 antibodies remain pivotal in advancing our understanding of intercellular communication mechanisms and their implications in disease.
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