| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | CN5A; PDE5; CGB-PDE |
| WB Predicted band size | 100 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PDE5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PDE5A抗体的参考文献概览(注：文献为示例，实际引用需核实最新数据库)：
1. **"Phosphodiesterase 5A expression in human cardiovascular tissues"**
*作者：Lin CS等*
摘要：通过免疫组化和Western blot分析，验证了PDE5A抗体在人类心脏和血管组织中的特异性，发现PDE5A在平滑肌细胞中高表达，提示其与心血管疾病的相关性。
2. **"Localization of PDE5A in rat penile tissue using a novel monoclonal antibody"**
*作者：Wang H等*
摘要：开发并验证了一种针对大鼠PDE5A的单克隆抗体，证实其在阴茎海绵体组织中的特异性定位，为勃起功能障碍的机制研究提供工具。
3. **"Altered PDE5A expression in pulmonary hypertension: Role of antibody-based detection"**
*作者：Kass DA等*
摘要：采用PDE5A抗体检测肺高压动物模型中的酶表达变化，发现疾病状态下PDE5A活性升高，支持靶向抑制治疗的病理基础。
4. **"Comparative analysis of PDE5A isoforms in human prostate using selective antibodies"**
*作者：Bender AT等*
摘要：利用亚型特异性抗体区分PDE5A的不同剪接变体，揭示了其在正常和增生前列腺组织中的分布差异。
建议通过PubMed或Google Scholar以关键词“PDE5A antibody application”检索最新研究，关注抗体验证方法(如敲除实验验证特异性)及疾病相关应用。
The PDE5A antibody is a crucial tool in biomedical research, targeting phosphodiesterase type 5A (PDE5A), an enzyme that hydrolyzes cyclic guanosine monophosphate (cGMP) to regulate cellular signaling. PDE5A is widely expressed in vascular smooth muscle, platelets, and the corpus cavernosum, playing a key role in cardiovascular function and erectile physiology. Its inhibition by drugs like sildenafil (Viagra) underscores its therapeutic relevance. Researchers use PDE5A antibodies to study the enzyme's expression, localization, and activity in tissues or cells, aiding investigations into conditions such as pulmonary hypertension, erectile dysfunction, and cancer, where PDE5A dysregulation is implicated.
These antibodies are typically validated for applications like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and enzyme-linked immunosorbent assays (ELISA). Specificity is ensured through knockout controls or peptide blocking experiments. Monoclonal antibodies offer high specificity, while polyclonal versions may detect multiple isoforms (PDE5A1. PDE5A2). Host species (rabbit, mouse) and clonality are selected based on experimental needs.
Commercial PDE5A antibodies are often accompanied by validation data, ensuring reproducibility in studies exploring PDE5A's role in cGMP-dependent pathways, smooth muscle relaxation, or drug resistance. Their utility extends to biomarker discovery and therapeutic target validation, bridging basic research and clinical applications. Proper controls and optimized protocols are critical to avoid cross-reactivity with other PDE family members.
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