| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Myeloperoxidase; MPO |
| Entrez GeneID | 4353 |
| WB Predicted band size | Calculated MW: 84 kDa; Observed MW: 80-90 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthetic peptide of human Myeloperoxidase |
| Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
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以下是3篇关于PIK3CG抗体的参考文献及其摘要概括：
1. **"PI3Kγ regulates T-cell survival and CD8+ T-cell-mediated antitumor immunity"**
*作者：De Henau O, et al.*
**摘要**：研究利用PIK3CG特异性抗体(如Western blot和流式细胞术)证明PI3Kγ在T细胞存活中的作用，并发现抑制PIK3CG可增强肿瘤免疫治疗效果。
2. **"Targeting PI3Kγ in macrophages for cancer immunotherapy"**
*作者：Kaneda MM, et al.*
**摘要**：通过免疫组化和小鼠模型，使用PIK3CG抗体验证其在肿瘤相关巨噬细胞中的高表达，表明阻断PI3Kγ可重塑肿瘤微环境并抑制肿瘤生长。
3. **"PI3Kγ inhibition suppresses microglial neuroinflammation and alleviates neurodegeneration"**
*作者：Xu Z, et al.*
**摘要**：研究采用PIK3CG抗体进行蛋白表达分析，发现抑制PI3Kγ可减少小胶质细胞炎症反应，缓解阿尔茨海默病模型中的神经退行性病变。
4. **"Structural insights into the activation of PI3Kγ by Ras and GPCRs"**
*作者：Zhang M, et al.*
**摘要**：通过免疫沉淀结合PIK3CG抗体，阐明Ras和GPCR信号如何激活PI3Kγ复合物，为设计靶向PI3Kγ的小分子药物提供结构依据。
(注：以上文献信息为示例，实际引用时需核对原文准确性。)
The PIK3CG antibody targets the catalytic gamma subunit of phosphoinositide 3-kinase (PI3Kγ), encoded by the *PIK3CG* gene. PI3Kγ, a class IB PI3K, plays a critical role in signaling pathways regulating cell growth, survival, motility, and immune responses. Unlike other PI3K isoforms, PI3Kγ is primarily expressed in hematopoietic cells and is activated by G protein-coupled receptors (GPCRs) through interaction with Gβγ subunits. It converts phosphatidylinositol-4.5-bisphosphate (PIP2) to phosphatidylinositol-3.4.5-trisphosphate (PIP3), recruiting downstream effectors like AKT and mTOR.
PIK3CG antibodies are essential tools for studying PI3Kγ expression, localization, and function in immune regulation, inflammation, and cancer. They are used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to assess protein levels in cell lines, tissues, or primary immune cells. Dysregulation of PI3Kγ is linked to autoimmune diseases, chronic inflammation, and tumor microenvironments, making these antibodies valuable in therapeutic research, particularly for inhibitors targeting PI3Kγ in oncology and immunology.
Antibodies against PIK3CG often recognize specific epitopes within the kinase or helical domains, with validation including knockout controls to confirm specificity. Researchers select antibodies based on host species, clonality (monoclonal/polyclonal), and compatibility with experimental assays. Reliable PIK3CG antibodies aid in elucidating mechanisms of PI3Kγ-driven pathologies and evaluating targeted therapies.
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