| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human UBE2N |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3-4条关于 **UBE2N抗体** 的参考文献及其简要摘要：
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1. **文献名称**：*UBE2N is required for K63-linked polyubiquitination and DNA damage tolerance*
**作者**：Hofmann, R.M., & Pickart, C.M.
**摘要**：该研究通过UBE2N抗体检测，发现UBE2N与UBE2V1形成复合物，特异性催化K63连接的泛素链合成，在DNA损伤修复和信号转导中起关键作用，并揭示了其与蛋白酶体降解途径的差异。
2. **文献名称**：*The Ubc13/UEV1A complex is required for TNFα-induced NF-κB activation*
**作者**：Wu, C.J., & Chen, Z.J.
**摘要**：利用UBE2N特异性抗体阻断实验，证明UBE2N(Ubc13)与UEV1A结合后，介导TRAF6的K63泛素化，进而激活TAK1和NF-κB通路，调控炎症反应和细胞存活。
3. **文献名称**：*UBE2N interacts with the Fanconi anemia core complex to promote DNA repair*
**作者**：Sato, K., et al.
**摘要**：通过免疫共沉淀(Co-IP)结合UBE2N抗体，发现UBE2N与Fanconi贫血通路核心蛋白FANCI/FANCD2协同作用，参与DNA链间交联修复，并依赖其泛素结合酶活性维持基因组稳定性。
4. **文献名称**：*Structural basis for UBE2N-UBE2V2 heterodimerization and function in RNF168-mediated ubiquitination*
**作者**：Alpi, A.F., et al.
**摘要**：通过UBE2N抗体的表位定位和晶体结构分析，揭示了UBE2N与UBE2V2的异源二聚化机制，及其在RNF168介导的染色质泛素化中的功能，为靶向DNA修复的癌症治疗提供依据。
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这些文献涵盖了UBE2N在泛素化、DNA修复、NF-κB信号通路及结构生物学中的关键作用，抗体多用于Western blot、Co-IP和功能阻断实验。
UBE2N, also known as UBC13. is a ubiquitin-conjugating enzyme (E2) critical for catalyzing Lys63 (K63)-linked ubiquitination, a non-degradative post-translational modification involved in DNA repair, NF-κB signaling, and immune regulation. It functions by partnering with UBE2V1/UEV1A to form a heterodimer that facilitates the assembly of K63-linked polyubiquitin chains on substrate proteins. These chains serve as scaffolds for recruiting downstream signaling components, such as kinases in the NF-κB pathway or DNA damage response machinery. Dysregulation of UBE2N is implicated in cancer, neurodegenerative diseases, and inflammatory disorders, making it a key target for mechanistic and therapeutic studies.
UBE2N antibodies are essential tools for detecting and analyzing UBE2N expression, localization, and function in various biological contexts. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study its role in cellular stress responses, immune signaling, and oncogenesis. Specific UBE2N antibodies can distinguish between free UBE2N and its complexes with UBE2V1. aiding in mechanistic investigations. Validated antibodies are critical to ensure specificity, as cross-reactivity with other E2 enzymes may occur. Research utilizing UBE2N antibodies has advanced understanding of its contributions to genome stability, inflammation, and disease pathways, highlighting its potential as a therapeutic target.
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