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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MCSF |
| Uniprot No | P09603 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-190aa |
| 氨基酸序列 | EEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYL KKAFLLVQYIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDK ACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQD VVTKPDCN |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
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Macrophage Colony-Stimulating Factor (M-CSF), also known as CSF-1. is a critical cytokine regulating the survival, proliferation, and differentiation of mononuclear phagocytes, including macrophages and their progenitors. Discovered in the 1970s, M-CSF binds to its receptor CSF1R (CD115) on target cells, activating downstream signaling pathways like PI3K/AKT and MAPK. This interaction is essential for maintaining tissue-resident macrophage populations and modulating immune responses.
Recombinant M-CSF (rM-CSF) emerged as a biotechnological advancement in the 1980s through genetic engineering. By expressing the M-CSF gene (located on human chromosome 1p13.3) in host systems like Escherichia coli, mammalian cells, or yeast, researchers achieved scalable production of bioactive protein. The recombinant form retains the native homodimeric structure (70-90 kDa glycosylated form or 40 kDa non-glycosylated variant) and biological activity while ensuring batch-to-batch consistency.
Clinically, rM-CSF has shown therapeutic potential in hematological recovery post-chemotherapy/radiation by stimulating myeloid cell production. It also enhances antitumor immunity by polarizing macrophages toward pro-inflammatory phenotypes and has been investigated in combination with checkpoint inhibitors. Emerging applications include tissue engineering (osteoclast regulation for bone repair) and antiviral research, given macrophages' role in pathogen defense.
Notably, rM-CSF serves as a vital research tool for studying macrophage biology, inflammatory diseases, and cancer microenvironment interactions. Its therapeutic use remains carefully balanced due to dual roles of macrophages in tumor progression and suppression. Ongoing studies focus on optimizing delivery systems and understanding context-dependent macrophage modulation.
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