| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/20-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ITR |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human GPR180 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是基于GPR180相关研究领域生成的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**：*GPR180 Antibody Characterization and Its Role in Vascular Smooth Muscle Cell Migration*
**作者**：Smith A, et al.
**摘要**：本研究验证了GPR180抗体的特异性，并发现GPR180通过调节细胞内钙信号通路影响血管平滑肌细胞迁移，提示其在动脉粥样硬化中的潜在作用。
2. **文献名称**：*Expression Profiling of GPR180 in Tumor Stroma Using a Novel Monoclonal Antibody*
**作者**：Chen L, et al.
**摘要**：开发了一种高特异性抗GPR180单克隆抗体，证实GPR180在肿瘤基质细胞中高表达，可能与肿瘤微环境中的血管生成相关。
3. **文献名称**：*GPR180 Antibody-Based Detection of Receptor Localization in Cardiac Fibroblasts*
**作者**：Yamamoto K, et al.
**摘要**：通过免疫荧光和Western blot分析，揭示了GPR180在心脏成纤维细胞膜上的定位，并发现其敲减可抑制纤维化相关基因表达。
4. **文献名称**：*GPR180 Interaction with β-Arrestin: Insights from Antibody-Mediated Blockade Experiments*
**作者**：Patel R, et al.
**摘要**：利用GPR180功能阻断抗体，证明其与β-arrestin的相互作用对GPCR内吞及下游MAPK信号通路激活至关重要。
**说明**：以上内容为领域知识模拟生成，实际文献需通过PubMed/Google Scholar等平台检索关键词“GPR180 antibody”“GPR180 function”获取。建议结合具体研究方向筛选文献(如肿瘤、心血管或信号机制)。
GPR180. also known as G-protein coupled receptor 180. is a member of the adhesion class of G-protein coupled receptors (GPCRs), a large family of cell surface proteins involved in signal transduction. Initially identified through genomic studies, GPR180 remains an understudied orphan receptor, meaning its endogenous ligand and precise physiological roles are not fully elucidated. It is characterized by a long extracellular N-terminal domain, a feature common to adhesion GPCRs, which may mediate cell-cell or cell-matrix interactions. Emerging research suggests GPR180 is expressed in various tissues, including the vascular system, lungs, and kidneys, with potential roles in angiogenesis, tissue remodeling, and metabolic regulation. Its involvement in diseases such as cancer, fibrosis, and cardiovascular disorders has been hypothesized but requires further validation.
GPR180 antibodies are essential tools for investigating the receptor's expression, localization, and function. These antibodies are typically developed against specific epitopes in the extracellular or intracellular domains of GPR180 and are validated for applications like Western blotting, immunohistochemistry, and flow cytometry. Due to the receptor's orphan status and low expression levels in many tissues, antibody specificity is critical to avoid cross-reactivity with other GPCRs. Recent studies using GPR180 antibodies have linked the receptor to signaling pathways involving cAMP and MAPK, suggesting its potential interplay with cellular proliferation and migration. However, the functional relevance of GPR180 in health and disease remains an active area of research, necessitating reliable antibody reagents for mechanistic exploration.
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