Mouse Erythropoietin,EPO ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04539m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠红细胞生成素(EPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPO含量。EPO即促红细胞生成素，是一种糖蛋白激素。它由肾脏分泌，主要作用是促进红细胞生成，调节机体的造血功能。在研究机制上，它能与红系祖细胞表面受体结合，促进其增殖、分化，增加红细胞数量，临床上常用于治疗肾性贫血等疾病。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。适用于基础科研中探索EPO在贫血机制、肿瘤代谢、造血调控等领域的生物学功能，或用于评估药物干预、基因修饰动物模型中的EPO表达变化，为相关疾病机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
EpoErythropoietin ELISA Kit
缩写：
EPO
Uniprot No.：
P07321
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/mL-2000 pg/mL
灵敏度：
7.81pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-98
1:2	Average %	97
	Range %	90-106
1:4	Average %	97
	Range %	92-104
1:8	Average %	94
	Range %	88-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	3.152	3.103	3.127	2.983
1000	2.534	2.667	2.601	2.456
500	1.741	1.673	1.707	1.563
250	0.982	0.963	0.973	0.828
125	0.519	0.532	0.525	0.381
62.5	0.286	0.275	0.281	0.137
31.25	0.209	0.198	0.203	0.059
0	0.146	0.143	0.144