Mouse Fibroblast growth factor 21(FGF21) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL008627MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠成纤维细胞生长因子21(FGF21)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的FGF21含量。FGF21是一种非典型的成纤维细胞生长因子，作为代谢调节剂，具有多重效应。研究发现，FGF21在肿瘤免疫治疗中发挥重要作用，通过改变CD8+T细胞胆固醇代谢，致使其功能耗竭，从而抑制抗肿瘤免疫应答。试剂盒检测范围为31.25 pg/ml-2000 pg/ml，灵敏度为7.81 pg/ml。用于探究代谢调控机制、疾病模型中FGF21表达动态变化、药物或营养干预对代谢通路的影响等基础研究，为体外实验提供稳定可靠的检测方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Fgf21Fibroblast growth factor 21 ELISA Kit; FGF-21 ELISA Kit
缩写：
FGF21
Uniprot No.：
Q9JJN1
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/ml -2000 pg/ml
灵敏度：
7.81 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	88
	Range %	84-92
1:4	Average %	100
	Range %	95-105
1:8	Average %	94
	Range %	90-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.066	2.229	2.148	2.043
1000	1.452	1.498	1.475	1.370
500	0.938	0.904	0.921	0.816
250	0.608	0.631	0.620	0.515
125	0.386	0.375	0.381	0.276
62.5	0.309	0.315	0.312	0.207
31.25	0.199	0.195	0.197	0.092
0	0.106	0.104	0.105