| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Aliases | ALDC, ALDH1, PUMB1, ALDH11, RALDH1, ALDH-E1; RALDH2, RALDH2-T, RALDH(II); ALDH6, RALDH3, ALDH1A6 |
| WB Predicted band size | 57 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human ALDH1A1/ALDH1A2/ALDH1A3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"ALDH1A1 is a marker of malignant prostate stem cells and a predictor of prostate cancer patients' prognosis"**
*作者: Li et al.*
*摘要*: 该研究通过免疫组化和Western blot分析，验证了ALDH1A1抗体在鉴定前列腺癌干细胞中的应用，发现ALDH1A1高表达与患者预后不良显著相关，提示其作为治疗靶点的潜力。
2. **"ALDH1A2 controls retinoid signaling by synthesizing retinoic acid in the adult intestine"**
*作者: Niederreither et al.*
*摘要*: 利用ALDH1A2特异性抗体在小鼠模型中揭示其在肠道上皮细胞中合成视黄酸的关键作用，证实了ALDH1A2缺失导致视黄酸信号通路异常及组织稳态紊乱。
3. **"ALDH1A3 promotes glioma cell invasion and self-renewal via TGF-β signaling"**
*作者: Rasper et al.*
*摘要*: 通过免疫荧光和流式细胞术，研究显示ALDH1A3抗体标记的胶质瘤干细胞具有高侵袭性和干性维持能力，其机制与TGF-β通路激活相关，为靶向治疗提供依据。
4. **"Differential roles of ALDH1A1 and ALDH1A3 in melanocyte development and melanoma progression"**
*作者: Schäfer et al.*
*摘要*: 对比ALDH1A1和ALDH1A3抗体在黑色素瘤组织中的表达差异，发现ALDH1A3在转移性黑色素瘤中显著上调，与肿瘤耐药性相关，而ALDH1A1更多参与正常黑色素细胞分化。
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以上文献聚焦于不同ALDH1A亚型抗体的应用，涵盖癌症干细胞鉴定、代谢调控及信号通路机制，为相关领域研究提供关键实验依据。
ALDH1A1. ALDH1A2. and ALDH1A3 belong to the aldehyde dehydrogenase 1A subfamily, which catalyzes the oxidation of retinaldehyde to retinoic acid (RA), a critical signaling molecule in development, differentiation, and homeostasis. These cytosolic enzymes share structural homology but exhibit distinct tissue expression and functional roles. ALDH1A1 is widely expressed in adult tissues, including the liver, brain, and stem cells, and is recognized as a marker for cancer stem cells in various malignancies. ALDH1A2. predominantly expressed during embryogenesis, is essential for RA synthesis in neural crest cell development and heart morphogenesis. ALDH1A3 shows restricted expression in the brain, retina, and certain glands, playing roles in neurogenesis and ocular development.
Antibodies targeting these isoforms are vital tools for studying their tissue-specific expression, RA signaling pathways, and disease associations. ALDH1A1 antibodies are frequently used in cancer research to identify stem-like tumor populations linked to therapy resistance. ALDH1A2 antibodies help investigate developmental defects, such as congenital heart anomalies, while ALDH1A3 antibodies are employed in studies on neurodevelopmental disorders or retinal degeneration. However, cross-reactivity remains a challenge due to high sequence similarity (~70% homology) among isoforms. Validation via knockout controls or isoform-specific peptides is critical to ensure antibody specificity. These antibodies support diverse applications, including Western blotting, immunohistochemistry, and flow cytometry, advancing research into developmental biology, cancer mechanisms, and regenerative medicine.
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