| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/2000 | Human,Mouse,Rat |
| Aliases | 5-LO, 5LPG, LOG5, 5-LOX |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human ALOX5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"Role of 5-lipoxygenase in the pathogenesis of colorectal cancer"**
- **作者**: Smith, J.R. et al.
- **摘要**: 研究通过免疫组化和Western blot分析ALOX5在结直肠癌组织中的高表达，证实其通过调控炎症介质促进肿瘤生长，抗体用于检测蛋白水平及定位。
2. **"ALOX5-mediated leukotriene biosynthesis drives neuroinflammation in Alzheimer’s disease"**
- **作者**: Chen, L. et al.
- **摘要**: 利用ALOX5抗体在小鼠模型和患者脑组织中发现其过度激活与神经炎症相关，抗体用于评估蛋白表达变化及病理关联。
3. **"Development of a monoclonal antibody targeting 5-lipoxygenase for therapeutic intervention in asthma"**
- **作者**: Gupta, S. et al.
- **摘要**: 报道新型ALOX5单克隆抗体的开发，通过抑制酶活性减少气道炎症，抗体应用于体外和动物模型中的药效评价。
4. **"ALOX5 polymorphisms and cardiovascular disease risk: A biomarker study"**
- **作者**: Wang, H. et al.
- **摘要**: 结合ELISA和基因分型技术，分析ALOX5蛋白水平与基因多态性对心血管疾病的影响，抗体用于血清样本定量检测。
(注：上述文献信息为示例性概括，实际引用需根据具体研究补充准确信息。)
ALOX5 (Arachidonate 5-Lipoxygenase) is a key enzyme in the biosynthesis of leukotrienes, lipid mediators involved in inflammatory and immune responses. It catalyzes the conversion of arachidonic acid to leukotriene A4 (LTA4), a precursor for pro-inflammatory leukotrienes like LTB4 and cysteinyl leukotrienes (CysLTs). ALOX5 plays a critical role in pathologies such as asthma, allergies, cardiovascular diseases, and cancer, particularly in processes like inflammation, cell proliferation, and metastasis.
ALOX5 antibodies are immunological tools designed to detect and quantify ALOX5 expression in research and diagnostic applications. These antibodies are widely used in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study tissue-specific expression, subcellular localization, and regulatory mechanisms of ALOX5 under physiological or pathological conditions. They are essential for investigating ALOX5's role in disease progression, such as its oncogenic potential in certain cancers or its contribution to chronic inflammatory disorders. Additionally, ALOX5 antibodies aid in evaluating the efficacy of ALOX5-targeted therapies, including inhibitors like zileuton, which block leukotriene synthesis. High specificity and validation across species (e.g., human, mouse, rat) are critical for reliable experimental outcomes, making antibody quality a priority in related studies.
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