| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | BCL2L4 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human BAX |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于BAX抗体的3篇示例参考文献(内容为模拟概括，建议通过学术数据库验证具体文献)：
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1. **文献名称**：*"BAX antibody validation in apoptotic signaling pathways"*
**作者**：Smith J, et al.
**摘要**：该研究系统评估了多种商业化BAX抗体的特异性及适用性，通过Western blot和免疫荧光技术验证了其在检测内源性BAX激活(如构象变化及线粒体转位)中的可靠性，为凋亡机制研究提供抗体选择指南。
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2. **文献名称**：*"Correlation of BAX expression with chemotherapy response in colorectal cancer using immunohistochemistry"*
**作者**：Chen L, et al.
**摘要**：利用BAX特异性抗体对结肠癌组织样本进行免疫组化分析，发现BAX高表达与奥沙利铂化疗敏感性显著相关，提示BAX可作为预测治疗反应的生物标志物。
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3. **文献名称**：*"BAX and BAK oligomerization in mitochondrial apoptosis: A flow cytometry-based approach"*
**作者**：Rodriguez D, et al.
**摘要**：开发了一种基于流式细胞术的方法，结合构象特异性BAX抗体，动态监测BAX/BAK寡聚化过程，揭示其在DNA损伤诱导的细胞凋亡中的时序性激活机制。
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**注**：以上为模拟摘要，实际文献需通过PubMed、Web of Science等平台检索关键词如“BAX antibody validation”、“BAX apoptosis detection”获取。推荐筛选近5年文献并关注高影响力期刊(如*Cell Death & Disease*、*Apoptosis*)。
**Background of BAX Antibodies**
BAX (BCL2-associated X protein) is a pro-apoptotic member of the BCL-2 protein family, playing a critical role in regulating mitochondrial apoptosis. Under cellular stress (e.g., DNA damage), BAX undergoes conformational changes, translocates to the mitochondrial outer membrane, and promotes permeabilization, leading to cytochrome *c* release and caspase activation. Dysregulation of BAX is implicated in cancer, neurodegenerative diseases, and resistance to therapies.
BAX antibodies are essential tools for detecting BAX expression, localization, and activation status in research. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Specific antibodies target distinct epitopes or post-translational modifications (e.g., activated BAX conformation). Validation often includes knockout controls to confirm specificity.
Commercial BAX antibodies vary in host species (rabbit, mouse), clonality (monoclonal/polyclonal), and applications. For example, Clone 3 is commonly used for total BAX detection, while Clone 6A7 recognizes conformationally active BAX. Researchers must select antibodies based on experimental needs, ensuring compatibility with sample preparation methods.
Overall, BAX antibodies are pivotal in studying apoptosis mechanisms, drug screening, and disease biomarker discovery, contributing to advancements in oncology and cell biology research.
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