| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MOBKL1B |
| Uniprot No | Q9H8S9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-216aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSFLFSSRSSKTFKPKKNIPEGSHQYELLK HAEATLGSGNLRQAVMLPEGEDLNEWIAVNTVDFFNQINMLYGTITEFCT EASCPVMSAGPRYEYHWADGTNIKKPIKCSAPKYIDYLMTWVQDQLDDET LFPSKIGVPFPKNFMSVAKTILKRLFRVYAHIYHQHFDSVMQLQEEAHLN TSFKHFIFFVQEFNLIDRRELAPLQELIEKLGSKDR |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与MOBKL1B(MOB kinase activator 1B)重组蛋白相关的文献摘要概览：
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1. **文献名称**: *"MOB1 mediated recognition of the STRN3:STRIPAK complex is required for proper cell cycle progression"*
**作者**: Xiong S, **et al.**
**摘要**: 研究解析了MOB1B重组蛋白与STRIPAK复合物核心组分STRN3的相互作用机制，发现MOB1B通过磷酸化依赖的构象变化调控Hippo信号通路，影响细胞周期进程与基因组稳定性。
2. **文献名称**: *"Structural basis for the phosphorylation-regulated interaction between the kinase Hippo and the scaffold protein Salvador"*
**作者**: **Hergovich A**, **et al.**
**摘要**: 通过重组表达纯化MOB1B与Hippo激酶复合物，结合晶体结构分析，揭示了MOB1B作为支架蛋白在Hippo-YAP信号通路中的构象调控作用，为肿瘤抑制机制提供结构生物学证据。
3. **文献名称**: *"Regulation of neuronal differentiation by the phosphorylation of MOB proteins"*
**作者**: **Praskova M**, **et al.**
**摘要**: 研究利用重组MOB1B蛋白进行体外磷酸化实验，发现其磷酸化状态通过调控Ndr激酶活性影响神经元分化过程，提示MOB1B在神经发育中的潜在功能。
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**备注**：MOBKL1B(MOB1B)多与Hippo信号通路相关，上述文献聚焦其重组蛋白在结构解析、磷酸化互作及细胞功能调控中的应用。若需具体实验方法或更多文献，建议通过PubMed/Google Scholar以“MOB1B recombinant protein”或“MOBKL1B signaling”为关键词进一步检索。
MOBKL1B (Mps One Binder Kinase Activator-Like 1B), also known as MOB1B, is a conserved regulatory protein belonging to the MOB (Mps One Binder) family. It plays a critical role in cellular signaling pathways, particularly the Hippo signaling pathway, which governs organ size control, tissue homeostasis, and tumor suppression. MOBKL1B functions as a co-activator of the core kinases LATS1/2 (Large Tumor Suppressor Kinase 1/2) by forming complexes with them, facilitating their phosphorylation and activation. This activation cascade ultimately regulates the transcriptional co-activators YAP/TAZ, influencing cell proliferation, apoptosis, and differentiation. Dysregulation of MOBKL1B is linked to cancer progression, developmental disorders, and tissue overgrowth.
Recombinant MOBKL1B protein is produced through heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical interactions, structural properties, and functional mechanisms. Purified recombinant MOBKL1B retains the ability to bind LATS1/2 and modulate Hippo pathway activity, making it a valuable tool for in vitro kinase assays, protein-protein interaction studies, and drug screening. Its applications extend to elucidating signaling crosstalk with other pathways, such as Wnt and Notch, and exploring therapeutic strategies targeting Hippo-related diseases. Research on MOBKL1B also contributes to understanding cell cycle regulation, mitotic exit, and centrosome dynamics. The recombinant form often includes tags (e.g., His, GST) for purification and detection, ensuring experimental reproducibility and specificity. Current studies focus on its post-translational modifications, tissue-specific roles, and potential as a biomarker or therapeutic target in cancers characterized by YAP/TAZ hyperactivity.
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