| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYL12B |
| Uniprot No | O14950 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-172aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMSSKKA KTKTTKKRPQ RATSNVFAMF DQSQIQEFKE AFNMIDQNRD GFIDKEDLHD MLASLGKNPT DAYLDAMMNE APGPINFTMF LTMFGEKLNG TDPEDVIRNA FACFDEEATG TIQEDYLREL LTTMGDRFTD EEVDELYREA PIDKKGNFNY IEFTRILKHG AKDKDD |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYL12B重组蛋白的3篇代表性文献，格式为文献名称、作者及摘要概括：
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1. **"Recombinant MYL12B regulates cytoskeletal dynamics in cancer cell migration"**
*Zhang L, et al. (2018)*
摘要：研究通过大肠杆菌表达系统纯化重组MYL12B蛋白，发现其通过调控肌动蛋白聚合促进肿瘤细胞迁移，并验证了其与RhoA信号通路的相互作用。
2. **"Structural and functional characterization of human MYL12B using recombinant protein expression"**
*Kim S, Park JH. (2020)*
摘要：利用昆虫细胞系统表达并解析MYL12B重组蛋白的晶体结构，揭示了其磷酸化位点对肌球蛋白ATP酶活性的调控机制，为靶向药物开发提供结构基础。
3. **"Development of a MYL12B-overexpressing cell model for studying chemoresistance"**
*Wang Y, et al. (2019)*
摘要：通过构建重组MYL12B稳定表达细胞系，证实其在结肠癌细胞中通过激活PI3K/AKT通路增强化疗耐药性，提示其作为潜在治疗靶点。
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注：以上文献为模拟示例，实际引用需查询PubMed或SciHub等平台获取真实文献。如需具体文献DOI或全文链接，建议补充关键词(如疾病模型、实验方法等)进一步筛选。
MYL12B (myosin light chain 12B) is a regulatory protein that plays a critical role in cellular contractility, motility, and cytoskeletal organization. It belongs to the myosin light chain family, specifically the regulatory light chain (RLC) subgroup, which interacts with myosin II heavy chains to modulate actomyosin ATPase activity and mechanical force generation. MYL12B is encoded by the MYL12B gene in humans and is expressed in various tissues, including smooth muscle and non-muscle cells. Its function is tightly regulated by phosphorylation, particularly at specific serine/threonine residues, which influences myosin II assembly and cellular processes like cytokinesis, cell adhesion, and migration.
Recombinant MYL12B protein is engineered for research and therapeutic applications to study its biochemical properties, interactions, and signaling pathways. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein retains functional domains necessary for binding myosin heavy chains and responding to upstream kinases such as Rho-associated kinase (ROCK) or myosin light chain kinase (MLCK). Purification tags (e.g., His-tag) are often added to facilitate isolation.
Studies using recombinant MYL12B have advanced understanding of its role in diseases, including cancer metastasis (via promoting invadopodia formation) and cardiovascular disorders linked to abnormal smooth muscle contraction. It also serves as a tool for screening kinase inhibitors or drugs targeting cytoskeletal dynamics. Structural analyses of recombinant MYL12B have elucidated conformational changes during phosphorylation, offering insights into mechanotransduction pathways. Overall, this protein is pivotal in both basic research and translational studies exploring cell mechanics and related pathologies.
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