| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/5000 | Human,Mouse,Rat |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human DOK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于DOK4抗体的虚构参考文献示例(基于学术文献常见结构模拟，非真实发表论文)：
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1. **文献名称**：*DOK4 Expression in Non-Small Cell Lung Cancer: A Prognostic Marker via EGFR Signaling*
**作者**：Chen L, et al.
**摘要**：本研究通过免疫组化及Western blot分析，发现DOK4蛋白在非小细胞肺癌组织中高表达，并与EGFR信号通路活化相关。使用特异性DOK4抗体证实其通过抑制Ras/MAPK通路减缓肿瘤进展，提示其作为潜在治疗靶点。
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2. **文献名称**：*Role of DOK4 in Leukemogenesis: Insights from Knockdown Models*
**作者**：Saito K, et al.
**摘要**：通过shRNA沉默DOK4基因并结合抗DOK4抗体的免疫沉淀技术，研究其在急性髓系白血病中的作用。结果显示DOK4缺失导致JAK-STAT信号异常激活，促进白血病细胞增殖，表明DOK4具有抑癌功能。
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3. **文献名称**：*DOK4 Antibody-Based Detection in Colorectal Cancer Stromal Cells*
**作者**：Gupta R, et al.
**摘要**：利用商业化DOK4单克隆抗体对结直肠癌样本进行免疫荧光分析，发现肿瘤微环境中成纤维细胞的DOK4表达上调，并通过旁分泌作用促进癌细胞侵袭，为靶向肿瘤基质提供新思路。
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注：以上内容为模拟文献，实际研究中需查阅真实数据库(如PubMed)获取具体信息。
**Background of DOK4 Antibody**
The DOK4 (Docking Protein 4) antibody is a tool used to study the DOK4 protein, a member of the DOK family of adaptor proteins involved in signal transduction. DOK proteins typically act as docking molecules, facilitating interactions between phosphorylated tyrosine kinase receptors (e.g., insulin or cytokine receptors) and downstream signaling effectors. DOK4. encoded by the *DOK4* gene, contains conserved domains such as a pleckstrin homology (PH) domain and a phosphotyrosine-binding (PTB) domain, enabling its role in regulating cellular processes like proliferation, differentiation, and survival.
DOK4 is particularly associated with signaling pathways involving Ras/MAPK and PI3K/Akt, and it has been implicated in developmental processes, including organogenesis and hematopoietic cell regulation. Dysregulation of DOK4 has been linked to diseases such as cancer, where it may act as a tumor suppressor or oncogene depending on context, as well as metabolic disorders.
The DOK4 antibody is utilized in research to detect and quantify DOK4 expression, assess its localization via techniques like Western blotting (WB) or immunohistochemistry (IHC), and investigate protein-protein interactions through immunoprecipitation (IP). Its specificity is validated using knockout controls or siRNA-based silencing. This antibody supports studies in cancer biology, developmental disorders, and metabolic dysregulation, aiding in the exploration of DOK4's mechanistic roles and therapeutic potential.
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