| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | BRK |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human PTK6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于PTK6抗体的参考文献及其摘要概括：
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1. **文献名称**：*PTK6 promotes insulin-like growth factor-induced proliferation of pancreatic ductal adenocarcinoma cells*
**作者**：Zheng Y, et al.
**摘要**：研究利用PTK6特异性抗体检测其在胰腺癌细胞中的表达，发现PTK6通过激活IGF-1R信号通路促进肿瘤增殖，抗体在免疫印迹和免疫组化中验证了其作为治疗靶点的潜力。
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2. **文献名称**：*Brk/PTK6 signaling in normal and cancer stem cells*
**作者**：Harvey AJ, Crompton MR.
**摘要**：通过PTK6抗体分析其在乳腺癌干细胞中的定位，发现PTK6通过调控Wnt/β-catenin通路维持干细胞特性，抗体在流式细胞术和免疫沉淀实验中用于功能研究。
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3. **文献名称**：*Development of a monoclonal antibody against PTK6 with diagnostic applications in colorectal cancer*
**作者**：Liu X, et al.
**摘要**：报道了一种新型PTK6单克隆抗体的开发，该抗体在结直肠癌组织芯片中显示出高特异性，可用于临床病理诊断及预后评估，并通过ELISA验证其灵敏度。
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**备注**：以上文献为虚拟示例，实际引用建议通过PubMed或Google Scholar以“PTK6 antibody”或“Brk kinase antibody”为关键词检索近期论文。
**Background of PTK6 Antibody**
PTK6 (Protein Tyrosine Kinase 6), also known as Brk (Breast Tumor Kinase), is an intracellular non-receptor tyrosine kinase belonging to the Src kinase family. Initially identified in metastatic melanoma, PTK6 is expressed in various epithelial tissues and plays dual roles in regulating cell signaling pathways involved in proliferation, differentiation, migration, and apoptosis. Structurally, it contains SH3. SH2. and catalytic kinase domains, enabling interactions with signaling molecules like adaptor proteins, receptors (e.g., EGFR, HER2), and transcription factors.
PTK6 is implicated in cancer progression, with elevated expression observed in breast, colon, prostate, and pancreatic cancers. Its oncogenic activity often involves promoting tumor cell survival, metastasis, and therapy resistance. However, context-dependent tumor-suppressive roles have also been reported, highlighting its complex regulatory mechanisms.
PTK6 antibodies are essential tools for studying its expression, localization, and function. They enable detection via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Research using PTK6 antibodies has revealed its post-translational modifications, interaction networks, and downstream targets (e.g., STAT3. β-catenin). These studies contribute to understanding PTK6's role in cancer biology and its potential as a therapeutic target or prognostic biomarker. Current efforts explore inhibitors targeting PTK6 kinase activity, with antibodies aiding in validating drug efficacy and mechanism of action in preclinical models.
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