| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | LIP8; PP1221 |
| WB Predicted band size | 101 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human CNTROB |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CNTROB抗体的3篇代表性文献及其摘要内容：
1. **文献名称**: *CNTROB promotes cell proliferation via APC/C-CDH1-regulated cell cycle protein stability*
**作者**: Hossain D, et al.
**摘要**: 该研究利用CNTROB抗体进行免疫沉淀和免疫荧光实验，发现CNTROB通过调控APC/C-CDH1复合体活性，影响细胞周期蛋白(如Cyclin B1)的稳定性，从而促进肿瘤细胞增殖和周期进程。
2. **文献名称**: *Centrobin interacts with Plk1 and regulates mitotic spindle organization*
**作者**: Li Y, et al.
**摘要**: 通过CNTROB抗体的共定位分析，研究发现CNTROB(Centrobin)与激酶Plk1直接结合，并在有丝分裂中参与纺锤体微管的形成，敲低CNTROB导致染色体排列异常和细胞分裂缺陷。
3. **文献名称**: *CNTROB expression correlates with poor prognosis in glioblastoma patients*
**作者**: Chen L, et al.
**摘要**: 该研究利用CNTROB抗体对胶质母细胞瘤组织进行免疫组化分析，发现CNTROB高表达与患者生存期缩短显著相关，提示其作为潜在预后标志物及治疗靶点的价值。
注：上述文献信息为示例性质，实际文献需通过数据库(如PubMed)检索确认。
The CNTROB (Centrobin) antibody is a research tool designed to target the Centrobin protein, a key centrosomal component involved in centriole duplication and cell cycle regulation. Encoded by the *CNTROB* gene in humans, Centrobin is predominantly localized to the daughter centriole, where it plays a critical role in maintaining centriole integrity and ensuring proper mitotic progression. Structurally, it contains coiled-coil domains that facilitate interactions with other centrosomal proteins like PCM1 and Pericentrin, contributing to centrosome maturation and microtubule organization.
CNTROB's function extends beyond structural support; it regulates the G1/S transition by influencing cyclin E/CDK2 activity, linking centrosome dynamics to cell cycle control. Dysregulation of CNTROB has been implicated in cancers, including breast and prostate cancer, where its overexpression correlates with tumor aggressiveness and genomic instability. Additionally, mutations or altered expression may contribute to neurodevelopmental disorders due to disrupted neuronal cell division.
The CNTROB antibody is widely used in techniques like immunofluorescence, Western blotting, and immunoprecipitation to study centrosome biology, centriole duplication mechanisms, and disease-associated anomalies. Its specificity enables researchers to visualize subcellular localization, assess protein expression levels, and explore interactions within the centrosomal network. As a vital reagent in cell biology and oncology research, this antibody aids in elucidating how centrosomal aberrations drive pathological processes, offering potential insights for therapeutic targeting.
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