| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | DCTN22; DCTN-22 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human DCTN3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DCTN3抗体的模拟参考文献示例(仅供参考，建议通过学术数据库核实最新文献)：
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1. **文献名称**: "DCTN3 as a potential biomarker in neurodegenerative diseases: Antibody development and validation"
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种高特异性DCTN3多克隆抗体，通过免疫印迹和免疫组化验证其在人类脑组织中的表达。结果表明，DCTN3在ALS患者神经元中表达显著降低，提示其可能与神经轴突运输功能障碍相关。
2. **文献名称**: "Structural insights into the dynactin complex: Role of DCTN3 subunit in microtubule binding"
**作者**: Lee H, et al.
**摘要**: 利用冷冻电镜技术和DCTN3特异性抗体，解析了动力蛋白复合物中DCTN3亚基的结构，发现其通过与微管结合蛋白相互作用调控细胞内物质运输，为相关疾病的分子机制提供了新见解。
3. **文献名称**: "DCTN3 antibody-based screening identifies dysregulation in cancer cell motility"
**作者**: Garcia R, et al.
**摘要**: 研究采用DCTN3单克隆抗体筛选多种癌细胞系，发现DCTN3表达水平与细胞迁移能力呈负相关，提示其可能通过影响动力蛋白复合物功能抑制肿瘤转移。
4. **文献名称**: "Immunohistochemical analysis of DCTN3 in peripheral neuropathies"
**作者**: Chen L, et al.
**摘要**: 通过免疫组化技术结合DCTN3抗体，分析了周围神经病变患者样本，发现DCTN3在施万细胞中异常聚集，可能参与髓鞘形成障碍的病理过程。
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**备注**：以上内容为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词"DCTN3 antibody"或"Dynactin subunit 3"获取。
The DCTN3 antibody targets the dynactin subunit 3 (DCTN3), a critical component of the dynactin protein complex. Dynactin, a multi-subunit complex, works in conjunction with cytoplasmic dynein to regulate microtubule-based intracellular transport processes, including organelle positioning, vesicle trafficking, and mitotic spindle organization. DCTN3. also known as p22. stabilizes the dynactin structure by linking the Arp1 filament to the shoulder/sidearm subcomplex. This interaction is essential for dynein-dynactin motor activity and cargo adaptor binding.
DCTN3 antibodies are widely used in research to study dynactin assembly, intracellular transport mechanisms, and dynein-mediated processes. They enable detection of DCTN3 expression levels, localization, and interactions in cellular models, contributing to insights into diseases linked to dynactin dysfunction, such as neurodegenerative disorders (e.g., amyotrophic lateral sclerosis, Perry syndrome) and cancer. Abnormal dynactin function can disrupt axonal transport in neurons or mitotic processes in dividing cells, driving pathological outcomes.
Commercial DCTN3 antibodies are typically validated for applications like Western blotting, immunofluorescence, and co-immunoprecipitation. Researchers also utilize these antibodies to explore DCTN3's role in viral infection mechanisms, as some pathogens hijack dynein-dynactin transport for intracellular trafficking. Overall, DCTN3 antibodies serve as vital tools for dissecting cytoskeletal dynamics and their implications in cellular health and disease. (298 words)
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