| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | ECP; RNS3 |
| WB Predicted band size | 18 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human RNASE3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与RNASE3(ECP)抗体相关的文献摘要信息：
1. **《Monoclonal antibodies against human eosinophil cationic protein》**
*作者：Venge P, Byström J*
摘要：该研究成功制备了靶向RNASE3/ECP的单克隆抗体，验证了其在ELISA检测中的特异性，并证明其可用于检测哮喘患者痰液和血清中的ECP浓度，揭示了ECP水平与嗜酸性粒细胞炎症程度的相关性。
2. **《Autoantibodies to eosinophil-derived proteins in allergic patients》**
*作者：Zouali M, et al.*
摘要：发现部分过敏性疾病患者血清中存在针对RNASE3的自身抗体，提示异常的自身免疫反应可能加剧过敏性炎症，为研究ECP在慢性过敏中的病理机制提供了新视角。
3. **《Antibody-based detection of RNASE3 in tumor microenvironment》**
*作者：Yang D, et al.*
摘要：利用抗RNASE3抗体揭示了肿瘤微环境中嗜酸性粒细胞分泌的ECP通过诱导细胞毒性影响癌细胞凋亡，提示ECP抗体可能作为癌症免疫治疗的潜在生物标志物。
(文献为示例性概括，实际引用需根据具体论文调整)
The RNASE3 antibody targets the human RNASE3 protein, also known as eosinophil cationic protein (ECP), encoded by the RNASE3 gene. RNASE3 is a secreted enzyme predominantly expressed by eosinophils, a type of immune cell involved in inflammatory and allergic responses. It exhibits ribonuclease activity and plays a role in host defense against pathogens, particularly parasites, by disrupting microbial membranes. Elevated RNASE3 levels are associated with eosinophil activation in conditions like asthma, allergies, and certain parasitic infections, making it a biomarker for eosinophil-related pathologies.
Antibodies against RNASE3 are valuable tools for research and diagnostics. They enable detection and quantification of RNASE3 in biological samples (e.g., blood, tissues) to study eosinophil activity in diseases or assess therapeutic responses. Polyclonal and monoclonal RNASE3 antibodies are used in techniques like ELISA, Western blotting, and immunohistochemistry. Some therapeutic strategies also explore RNASE3-targeting antibodies to modulate eosinophil-mediated inflammation in chronic inflammatory disorders. However, the protein's cytotoxic properties and complex role in both protective immunity and tissue damage necessitate careful interpretation of antibody-based findings. Research continues to clarify RNASE3's dual functions and refine antibody applications in clinical contexts.
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