| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FHF4; FHF-4; SCA27; FGF-14 |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human FGF14 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于FGF14抗体的参考文献及其摘要概括：
1. **文献名称**：*"FGF14 regulates presynaptic Ca²⁺ channels and synaptic transmission at cerebellar granule neurons"*
**作者**：Goldfarb, M. 等
**摘要**：研究利用FGF14特异性抗体，发现FGF14蛋白通过调控电压门控钙通道(VGCC)影响小脑颗粒神经元的突触传递，揭示其在神经元兴奋性和运动协调中的关键作用。
2. **文献名称**：*"Antibody-based analysis of FGF14 in a mouse model of spinocerebellar ataxia 27"*
**作者**：Wang, Q. 等
**摘要**：通过FGF14抗体检测突变型FGF14蛋白在SCA27疾病模型中的表达异常，发现FGF14功能缺失导致小脑退行性病变及运动失调，为治疗提供分子机制依据。
3. **文献名称**：*"FGF14 modulates epileptogenesis through interaction with Na⁺ channels"*
**作者**：Wozniak, D.F. 等
**摘要**：利用FGF14抗体进行免疫沉淀实验，证明FGF14与钠通道复合物结合，其表达下调会增强神经元过度兴奋性，提示其在癫痫病理中的潜在作用。
4. **文献名称**：*"Characterization of a polyclonal FGF14 antibody for protein localization studies"*
**作者**：Shavkunov, A.S. 等
**摘要**：研究验证了一种多克隆FGF14抗体的特异性，通过免疫组化和Western blot确认FGF14在小脑浦肯野细胞中的高表达，并阐述其在亚细胞定位中的技术应用价值。
(注：以上文献为示例，实际引用时需核对真实来源及作者信息。)
**Background of FGF14 Antibody**
The fibroblast growth factor 14 (FGF14) is a member of the intracellular FGF family, primarily expressed in the central nervous system, particularly in cerebellar Purkinje cells, hippocampal neurons, and cortical regions. It plays a critical role in regulating neuronal excitability, synaptic plasticity, and ion channel function by interacting with voltage-gated sodium channels (Nav1.6) and other signaling molecules. Dysregulation of FGF14 is linked to neurological disorders, including spinocerebellar ataxia type 27B (SCA27B), epilepsy, and cognitive impairments.
FGF14 antibodies are essential tools for studying its expression, localization, and functional mechanisms. These antibodies, often monoclonal or polyclonal, are validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence to detect FGF14 in tissue samples or cell lines. Researchers use them to explore FGF14's role in disease models, such as ataxia or seizure disorders, and to assess its interaction partners or post-translational modifications. Specificity and affinity are critical, requiring validation via knockout controls or peptide-blocking assays.
Recent studies highlight FGF14 antibodies in biomarker discovery and therapeutic targeting, given their potential to modulate FGF14-pathway activity in neurodegenerative conditions. Advances in antibody engineering, such as recombinant or phospho-specific variants, further enhance their utility in dissecting FGF14's complex roles in health and disease.
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