| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | GBP-5 |
| WB Predicted band size | 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Fusion protein of human GBP5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GBP5抗体的3篇参考文献示例(注：文献信息为假设性概括，仅供格式参考)：
1. **"Guanylate-binding protein 5 licenses caspase-11 activation for host defense"**
*作者：Santos JC, et al.*
*摘要*：本研究揭示了GBP5在细菌感染中通过调控caspase-11激活炎症小体的机制，利用GBP5特异性抗体证实其在巨噬细胞中的定位及与炎症复合物的相互作用。
2. **"GBP5 promotes NLRP3 inflammasome activation and pyroptosis in response to cytoplasmic LPS"**
*作者：Shenoy AR, et al.*
*摘要*：通过免疫沉淀和Western blot实验(使用抗GBP5抗体)，作者发现GBP5直接结合脂多糖(LPS)，并促进NLRP3炎症小体活化，阐明其在先天免疫中的关键作用。
3. **"Dysregulation of GBP5 in tumor microenvironment promotes immune evasion"**
*作者：Wang L, et al.*
*摘要*：该研究利用免疫组化(GBP5抗体)分析癌症患者组织样本，发现GBP5低表达与肿瘤免疫逃逸相关，提示其作为潜在免疫治疗靶点的价值。
注：实际文献需通过学术数据库检索确认，以上内容为示例性模拟。
The guanylate-binding protein 5 (GBP5) is a member of the interferon (IFN)-inducible GTPase family, which plays critical roles in innate immunity and inflammatory responses. GBP5 is primarily expressed in immune cells, such as macrophages and endothelial cells, and its expression is strongly upregulated by proinflammatory cytokines (e.g., IFN-γ) or pathogen-associated molecular patterns (PAMPs). Structurally, GBP5 contains a conserved N-terminal GTPase domain and a C-terminal helical domain, enabling its oligomerization and participation in host defense mechanisms.
Functionally, GBP5 is implicated in regulating inflammasome activation, antimicrobial defense, and cytokine secretion. Studies suggest it restricts intracellular pathogens (e.g., bacteria, viruses) by disrupting pathogen membranes or modulating immune signaling pathways. For example, GBP5 interacts with components of the NLRP3 inflammasome to enhance caspase-1 activation, promoting IL-1β processing. It also restricts HIV-1 infection by interfering with viral capsid assembly.
GBP5 antibodies are essential tools for investigating its expression, localization, and mechanistic roles in immune regulation. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study GBP5’s involvement in diseases such as sepsis, autoimmune disorders, and infections. Dysregulation of GBP5 has been linked to chronic inflammation and cancer, highlighting its therapeutic and diagnostic potential. Research on GBP5 continues to uncover its dual roles in pathogen defense and immune homeostasis.
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