| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/30-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EDF; FRP |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human INHBA |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于LEP(瘦素)抗体的3篇示例参考文献(注：文献为示例性质，实际引用需核实真实来源)：
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1. **文献名称**：*Development of a High-Specificity Leptin Antibody for Accurate Serum Leptin Measurement*
**作者**：Zhang Y, et al.
**摘要**：本研究报道了一种新型单克隆LEP抗体的开发，通过噬菌体展示技术筛选获得。该抗体对瘦素蛋白(LEP)具有高亲和力和特异性，可避免与瘦素受体或其他代谢激素的交叉反应。实验验证了其在ELISA和Western blot中的应用，为肥胖相关研究提供了可靠检测工具。
2. **文献名称**：*Leptin Expression in Adipose Tissue: A Comparative Study Using Commercial LEP Antibodies*
**作者**：Smith J, et al.
**摘要**：文章比较了5种市售LEP抗体的性能，通过免疫组化(IHC)和流式细胞术分析其在人脂肪组织中的检测效果。结果显示不同抗体的灵敏度和特异性差异显著，其中抗体Clone#4B11在区分瘦素异构体方面表现最优，强调了抗体选择对实验结果可靠性的影响。
3. **文献名称**：*LEP Antibody-Based Biosensor for Real-Time Monitoring of Leptin in Metabolic Disorders*
**作者**：Brown K, et al.
**摘要**：研究团队构建了一种基于LEP抗体的电化学生物传感器，可动态检测血清瘦素水平。该传感器在小鼠模型和临床样本中验证了高精度(检测限0.1 ng/mL)，揭示了瘦素昼夜节律波动与胰岛素抵抗的关联，为代谢综合征研究提供了新工具。
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建议通过PubMed或Google Scholar以关键词“Leptin antibody”、“LEP immunoassay”或“anti-LEP monoclonal antibody”检索真实文献，重点关注抗体开发、验证或应用类研究。
LEP antibodies are immunological tools designed to target leptin, a hormone encoded by the LEP gene. Leptin, primarily secreted by adipose tissue, regulates energy balance by suppressing appetite and promoting energy expenditure through interactions with hypothalamic receptors. Discovered in 1994. leptin became a focal point in obesity and metabolic research due to its role in signaling satiety and modulating fat storage. Dysregulation of leptin signaling—linked to leptin deficiency or resistance—is associated with obesity, diabetes, and metabolic disorders.
LEP antibodies are critical for both research and diagnostics. They enable quantification of leptin levels in serum or tissue samples via techniques like ELISA, Western blotting, or immunohistochemistry. Such applications help elucidate leptin expression patterns, receptor interactions, and mutations in metabolic diseases. Additionally, these antibodies aid in studying leptin's broader physiological roles, including immune regulation, reproduction, and bone metabolism.
Commercial LEP antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes of human or murine leptin. Validation for specificity and sensitivity is crucial, as cross-reactivity with related proteins may yield false results. Recent advancements include recombinant monoclonal antibodies with enhanced consistency. Beyond research, LEP antibodies hold potential in therapeutic development, such as neutralizing autoantibodies in rare leptin-related disorders or optimizing leptin sensitizers for obesity treatment. Their utility underscores leptin's enduring significance in metabolic pathophysiology.
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