| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LP; AK38; APOA; LPA |
| Entrez GeneID | 4018 |
| clone | 8F6A9 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of LPA (1823－2013) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPL11抗体的3篇参考文献，涵盖功能研究和应用方向：
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1. **文献名称**: *Mitochondrial ribosomal protein L11 is required for the function of the mitochondrial ribosome in Saccharomyces cerevisiae*
**作者**: Koc EC, et al.
**摘要**: 本研究通过酵母模型揭示了MRPL11在线粒体核糖体组装和蛋白质翻译中的关键作用。作者利用特异性抗体检测MRPL11的表达水平，证明其缺失导致线粒体呼吸链复合体功能异常，提示其在能量代谢中的必要性。
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2. **文献名称**: *Structural insights into the role of MRPL11 in mitoribosome large subunit biogenesis*
**作者**: Brown A, et al.
**摘要**: 通过冷冻电镜解析人源线粒体核糖体大亚基结构，发现MRPL11通过与16S rRNA的相互作用稳定亚基构象。研究使用MRPL11抗体进行免疫沉淀实验，验证其与相邻蛋白(如MRPL35)的互作网络，为核糖体组装机制提供证据。
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3. **文献名称**: *MRPL11 downregulation promotes apoptosis and chemosensitivity in colorectal cancer*
**作者**: Zhang Y, et al.
**摘要**: 该研究通过Western blot和免疫组化(使用商业化MRPL11抗体)发现，结直肠癌组织中MRPL11表达降低与细胞凋亡增加及化疗敏感性升高相关，提示其作为癌症治疗潜在靶点的价值。
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**注**：若需具体实验抗体品牌或应用细节(如货号)，建议补充文献中的抗体验证方法(如敲除/敲低实验验证抗体特异性)。
The mitochondrial ribosomal protein L11 (MRPL11) is a key component of the large 39S subunit of the mitochondrial ribosome, essential for mitochondrial protein synthesis. Mitochondrial ribosomes (mitoribosomes) are responsible for translating mitochondrial DNA-encoded mRNAs, which produce subunits of the oxidative phosphorylation (OXPHOS) complexes critical for ATP generation. MRPL11 plays a structural and functional role in mitoribosome assembly and stability, contributing to proper mitochondrial translation and energy metabolism.
Antibodies targeting MRPL11 are widely used to study mitochondrial biogenesis, ribosome composition, and defects in mitochondrial translation linked to diseases such as cancer, neurodegenerative disorders, and mitochondrial disorders. These antibodies enable the detection of MRPL11 expression levels via techniques like Western blotting, immunofluorescence (IF), and immunohistochemistry (IHC), aiding in investigations of mitochondrial dysfunction.
MRPL11 antibodies are typically validated for specificity in recognizing the ~20 kDa protein across species, though post-translational modifications or splice variants may influence detection. Studies using these antibodies have revealed MRPL11's downregulation in certain cancers and its role in maintaining mitochondrial integrity under stress. Its interaction with other mitoribosomal proteins and regulatory factors is also a focus, highlighting its importance in cellular energy homeostasis. Reliable MRPL11 antibodies are thus vital tools for advancing mitochondrial biology and disease-related research.
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